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Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE134299 | GEO | 2020/06/01

REPOSITORIES: GEO

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Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells.

Project description:Chromatin accessibility of a promoter is fundamental in regulating transcriptional activity. The histone variant H2A.Z has been shown to contribute to this regulation, but its role has remained poorly understood. Here, we prepare high-depth maps of the position and accessibility of H2A.Z-containing nucleosomes for all human Pol II promoters in epithelial, mesenchymal and isogenic cancer cell lines. We find that, in contrast to the prevailing model, many different types of active and inactive promoter structures are observed that differ in their nucleosome organization and sensitivity to MNase digestion. Key aspects of an active chromatin structure include positioned H2A.Z MNase resistant nucleosomes upstream or downstream of the TSS, and a MNase sensitive nucleosome at the TSS. Furthermore, the loss of H2A.Z leads to a dramatic increase in the accessibility of transcription factor binding sites. Collectively, these results suggest that H2A.Z has multiple and distinct roles in regulating gene expression dependent upon its location in a promoter.

| S-EPMC8100287 | biostudies-literature

Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (RNA-seq)

Project description:We employed an MNase-Transcription Start Site Sequence Capture method to map and determine the accessibility of all nucleosomes, including those that contain H2A.Z, at high coverage for all human Pol II promoters. We uncovered unexpected features of nucleosomal organization in epithelial cells and following transition to a mesenchymal and malignant state. In contrast to the prevailing model, we observe many different types of active and inactive promoter structures that differ in their nucleosome organization and sensitivity to MNase digestion. Further, we found that H2A.Z has an important role in the assembly of repressed promoters into an inaccessible state. Finally, we uncovered a new promoter type in which a stable H2A.Z nucleosome occupies the TSS of an active promoter.

2020-06-01 | GSE134298 | GEO

Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (Mnase-seq & ChIP-seq)

2020-06-01 | GSE134297 | GEO

Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells

Project description:Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells

| PRJNA554663 | ENA

Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (RNA-seq)

Project description:Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (RNA-seq)

| PRJNA554665 | ENA

R loops regulate promoter-proximal chromatin architecture and cellular differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-09-30 | GSE67584 | GEO

Coordinated Chromatin Remodeling induced by Demethylation requires SRCAP mediated H2A.Z exchange

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-01-01 | E-GEOD-26685 | biostudies-arrayexpress

H2A.Z.1 mono-ubiquitylation antagonizes BRD2 to maintain poised chromatin in ESCs

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-01-21 | E-GEOD-53208 | biostudies-arrayexpress

Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-07 | E-GEOD-59783 | biostudies-arrayexpress

Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (Mnase-seq & ChIP-seq)

Project description:Multiple roles of H2A.Z in regulating promoter chromatin architecture in human cells (Mnase-seq & ChIP-seq)

| PRJNA554664 | ENA

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