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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE134798 | GEO | 2020/07/07

REPOSITORIES: GEO

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (BLISS)

Project description:The depletion of HMGB1 reduces the deposition of histones on naked DNA (Celona et al, 2011). in primary MEFs and yeast. We verified that the downmodulation of HMGB1 decreases histone level also in a model of murine mesothelioma. HMGB1 was KD in Murine Mesothelioma AB22 cells by the expression of a shRNA. Nucleosome positioning and occupancy were tested by ATAC-seq. Since a reduced amount of nucleosomes increase the cell sensitivity to gamma irradiatio, we tested the number and position of breaks using BLISS-seq.

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells [BLISS human]

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (ATAC-seq)

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (RNA-seq)

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells

Project description:Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (BLISS)

Project description:Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (BLISS)

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells [BLISS human]

Project description:Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells [BLISS human]

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (ATAC-seq)

Project description:Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (ATAC-seq)

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Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (RNA-seq)

Project description:Nucleosomes protect DNA from irradiation-induced double strand breaks in living cells (RNA-seq)

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Cellular responses to DNA double-strand breaks after low-dose gamma-irradiation.

Project description:DNA double-strand breaks (DSBs) are a serious threat to genome stability and cell viability. Although biological effects of low levels of radiation are not clear, the risks of low-dose radiation are of societal importance. Here, we directly monitored induction and repair of single DSBs and quantitatively analyzed the dynamics of interaction of DNA repair proteins at individual DSB sites in living cells using 53BP1 fused to yellow fluorescent protein (YFP-53BP1) as a surrogate marker. The number of DSBs formed was linear with dose from 5 mGy to 1 Gy. The DSBs induced by very low radiation doses (5 mGy) were repaired with efficiency similar to repair of DSBs induced at higher doses. The YFP-53BP1 foci are dynamic structures: 53BP1 rapidly and reversibly interacted at these DSB sites. The time frame of recruitment and affinity of 53BP1 for DSB sites were indistinguishable between low and high doses, providing mechanistic evidence for the similar DSB repair after low- and high-dose radiation. These findings have important implications for estimating the risk associated with low-dose radiation exposure on human health.

| S-EPMC2709554 | biostudies-literature

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