Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

ABSTRACT: Purpose: After infection there was significant decrease in the lifespan of mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated which have role in lifespan Conclusions: This study showed the role of pathogenic infection in the life span modulation

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE137112 | GEO | 2020/06/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

Project description:Purpose: After infection there was dendrite degeneration in the mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated. Conclusions: This study showed the role of pathogenic infection in the life span modulation

2024-10-22 | GSE186478 | GEO

RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

Project description:RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

| PRJNA564575 | ENA

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA

Project description:Five biological repeats of P.aeruginosa PA14 and five biological repeats of P.aeruginosa PA14 metR::phoA were grown in liquid BM2 swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, and 0.1% (wt/vol) casamino acids. Microarray experiments were done using microarray slides and protocols from TIGR on samples taken from mid-log phase (OD600 0.4-0.5)cells.

2009-08-20 | E-FPMI-19 | biostudies-arrayexpress

Transcription profiling of P.aeruginosa PA14 and P.aeruginosa PA14 metR::phoA grown on swarming plates

Project description:Four biological repeats of P.aeruginosa PA14 and four biological repeats of P.aeruginosa PA14 metR::phoA were grown in BM2 swarming media plates containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids and 0.5% agar. Microarray experiments were done using microarray slides and protocols from TIGR on samples harvested from the dendritic swarm colony growth, 2 to 3 mm of the swarm zone edge.

2009-08-20 | E-FPMI-20 | biostudies-arrayexpress

Quantitative Analysis of Worms after P. aeruginosa Infection

Project description:To clarify the mechanisms of innate immune responses mediated by epithelial barrier disruption. Reveal the host’s broad transcriptional response to P. aeruginosa infection during disruption of the intestinal barrier. We used RNA-Seq analysis to study the transcriptomic profiles of wt worms were exposed to either P. aeruginosa PA14 or E. coli OP50 for 24 hours, and the transcriptomic profiles of egl-44(MT2247) mutants fed E. coli OP50 for 24 hours. Differential expression analysis was performed by comparing wt worms exposed to PA14 versus wt worms exposed to E. coli OP50, and egl-44(MT2247) mutants fed E. coli OP50 versus WT worms fed E. coli OP50.

2018-10-11 | GSE121091 | GEO

Quantitative Analysis of Wild Type worms and vit-5 RNAi worms Transcriptomes

Project description:Purpose: To identify the mechanism underlying reduced vitellogenin accumulation regulates the reproductive span, we used RNA-Seq analysis to study the transcriptional profiles Wild Type worms and vit-5 RNAi worms.

2021-08-20 | GSE182330 | GEO

C. elegans gene expression in healthy and PA14-infected wild-type and fshr-1 mutant worms

Project description:Analysis of gene expression in worms exposed to PA14 for 4 hours. Worms used were wild-type or fshr-1(ok778) mutants. Comparisons allowed determination of fshr-1-dependent gene expression.

2015-09-30 | GSE72029 | GEO

RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

Project description:RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

| PRJNA774046 | ENA

Gene expression in the small intestine of mice colonized with Pseudomonas aeruginosa

Project description:Celiac disease (CeD) is a food sensitivity characterized by a breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the underlying mechanisms are incompletely understood. To evaluate a functional role of bacterial proteases in CeD, we colonized germ-free or clean SPF mice with P.aeruginosa PA14 or P.aeruginosa PA14 disrupted in lasB gene (elastase). We show gluten-independent, PAR-2 mediated upregulation of inflammatory pathways by LasB in C57BL/6 mice without villus blunting. In mice expressing CeD risk genes, P. aeruginosa elastase synergized with gluten to induce more severe inflammation that was associated with moderate villus blunting.

2019-02-01 | GSE125983 | GEO

Worms

Project description:Worms Raw sequence reads

| PRJNA1008695 | ENA