Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

ABSTRACT: RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

PROVIDER: PRJNA774046 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16553205_1.fastq.gz	Fastqsanger.gz
	SRR16553205_2.fastq.gz	Fastqsanger.gz
	SRR16553206_1.fastq.gz	Fastqsanger.gz
	SRR16553206_2.fastq.gz	Fastqsanger.gz
	SRR16553207_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

Project description:RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

| PRJNA564575 | ENA

RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

Project description:Purpose: After infection there was significant decrease in the lifespan of mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated which have role in lifespan Conclusions: This study showed the role of pathogenic infection in the life span modulation

2020-06-24 | GSE137112 | GEO

RNA Sequencing of C. elegans control and mutant worms after 24 hour P. aeruginosa infection

Project description:Purpose: After infection there was dendrite degeneration in the mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated. Conclusions: This study showed the role of pathogenic infection in the life span modulation

2024-10-22 | GSE186478 | GEO

C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

Project description:Transcriptional profiling of C. elegans worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the nematode Pristionchus pacificus (separate data-sets on a custom microarray platform designed by us and manufactured by Agilent) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Total samples from both experiments 1 and 2 = 8.

2011-07-27 | E-GEOD-30977 | biostudies-arrayexpress

C. elegans gene expression in response to acute Pseudomonas aeruginosa infection (Part 1) and recovery (Part 2) by Neomycin/Streptomycin treatment

Project description:(Part 1) Gene expression profiles of C. elegans in response to P. aeruginosa. Synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours. These synchronized young adult (YA) animals were subsequently exposed to P. aeruginosa PA14 for 4 hours. 25dC. (Part 2) Gene expression profiles of C. elegans in response to Neomycin/Streptomycin-mediated recovery after a 4 hour exposure to P. aeruginosa. Synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours. These synchronized young adult (YA) animals were subsequently exposed to P. aeruginosa PA14 for 4 hours and then treated with Neomycin and shifted to E. coli OP50 plus Streptomycin plates for 6, 12, or 24 hours to resolve the infection. As a control for Neomycin/Streptomycin exposure, synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours and either shifted to fresh E. coli OP50 plates for 6 hours or treated with Neomycin and shifted to E. coli OP50 plus Streptomycin plates for 6 hours. 25dC.

2016-08-11 | GSE81592 | GEO

C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

2011-07-28 | GSE30977 | GEO

P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

Project description:Transcriptional profiling of P. pacificus worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the C. elegans (see NCBI GEO series GSE30977) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 3 biological replicates for each condition, including 1 dye-swaps. Total samples from both experiments 1 and 2 = 7.

2011-09-02 | E-GEOD-31861 | biostudies-arrayexpress

Quantitative Analysis of Worms after P. aeruginosa Infection

Project description:To clarify the mechanisms of innate immune responses mediated by epithelial barrier disruption. Reveal the host’s broad transcriptional response to P. aeruginosa infection during disruption of the intestinal barrier. We used RNA-Seq analysis to study the transcriptomic profiles of wt worms were exposed to either P. aeruginosa PA14 or E. coli OP50 for 24 hours, and the transcriptomic profiles of egl-44(MT2247) mutants fed E. coli OP50 for 24 hours. Differential expression analysis was performed by comparing wt worms exposed to PA14 versus wt worms exposed to E. coli OP50, and egl-44(MT2247) mutants fed E. coli OP50 versus WT worms fed E. coli OP50.

2018-10-11 | GSE121091 | GEO

The gene expression of wild-type worms and kin-1(ok338) mutant worms exposing Salmonella entericaSL1344 at 24 hours

Project description:PKA/KIN-1 pathway is required for innate immunity in C. elegans, however, the downstream signaling is largely unclear. To gain further insight into mechanism underlying the promoted immunity of PKA/KIN-1 pathway, we performed genome microarrays of wild-type and kin-1(ok338) worms infection by Salmonella entericSL1344, compared with the controls wild-type fed E.coli OP50

2017-03-03 | GSE86342 | GEO

24-hour transcriptome profile in the murine VTA

Project description:24-hour transcriptome profile in the murine VTA

| PRJNA517615 | ENA