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Gene expression change caused by CYS4 alleles

ABSTRACT: Each strain was grown overnight then diluted in fresh YPD to 10% v/v and grown for 3 hours at 30'C. Total RNA was isolated using an RNeasy mini kit, reverse transcribed, labeled with either Cy3 or Cy5, and hybridized to epoxy-coated slides with 6388, 70mer oligos (Qiagen-Operon). After an overnight hybridization, microarrays were scanned using a ScanArray Express laser scanner.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE14102 | GEO | 2008/12/30

SECONDARY ACCESSION(S): PRJNA111189

REPOSITORIES: GEO

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Gene expression change caused by CYS4 alleles

Project description:Each strain was grown overnight then diluted in fresh YPD to 10% v/v and grown for 3 hours at 30'C. Total RNA was isolated using an RNeasy mini kit, reverse transcribed, labeled with either Cy3 or Cy5, and hybridized to epoxy-coated slides with 6388, 70mer oligos (Qiagen-Operon). After an overnight hybridization, microarrays were scanned using a ScanArray Express laser scanner. Reference, a pool of total RNA from all samples, was used to contrast with each sample. Dye-swap was performed for one of the three replicates of each strain, where Cy3 instead of Cy5 was used to label the reference RNA.

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