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Shear stress-induced long non-coding RNA, NIMBUSS, is required for the KLF2-mediated gene expression profile associated with vascular homeostasis II

ABSTRACT: To investigate the regulation of the long non-coding RNA (lncRNA) transcriptome of endothelial cells in response to shear stress, gene expression profiling was performed using a microarray for both protein-coding genes and lncRNAs. The expression of known flow-induced protein coding genes, eNOS, KLF2, and KLF4, were quantified using real-time PCR. Identifying lncRNAs that are up-regulated or down-regulated in the presence or absence of laminar flow will provide insight into gene regulatory mechanisms that contribute to vascular homeostasis or disease.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142373 | GEO | 2024/12/01

REPOSITORIES: GEO

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Shear stress-induced long non-coding RNA, NIMBUSS, is required for the KLF2-mediated gene expression profile associated with vascular homeostasis I

Project description:The endothelial cell (EC) response to shear stress is partly mediated by the transcription factor KLF2, which is preferentially activated by laminar flow and promotes an atheroprotective phenotype. The up-regulation of KLF2 elicits anti-inflammmatory, anti-coagulant, and pro-vasodilatory processes. To investigate the function of the flow-induced long non-coding RNA (lncRNA) NIMBUSS, we performed loss-of-function studies using siRNA for NIMBUSS in ECs overexpressing KLF2.

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Angiogenic patterning by STEEL, an endothelial-enriched long noncoding RNAAngiogenic patterning by STEEL, an endothelial-enriched long noncoding RNA

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KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium

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2011-02-01 | E-GEOD-23289 | biostudies-arrayexpress

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2009-06-01 | E-GEOD-13712 | biostudies-arrayexpress

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2022-08-19 | GSE211662 | GEO

Comparison of STEEL siRNA-treated and control siRNA-treated human endothelial cells

Project description:To determine the role of STEEL in endothelial cell (EC) gene regulation, gene expression analysis was conducted on control and STEEL siRNA-treated human dermal microvascular endothelial cells (HMVECs) and human umbilical vein endothelial cells (HUVECs). A total of 225 protein-coding genes were downregulated and 80 were upregulated when both EC types were grouped for analysis. In HMVEC alone, 544 protein-coding genes were downregulated and 218 were upregulated. In HUVEC alone, 177 protein-coding genes were downregulated and 125 were upregulated. Prominently, STEEL siRNA depletion results in the downregulation of two notable protein-coding genes, eNOS and KLF2, which are modulated in ECs subjected to continuous laminar shear stress.

2018-03-30 | GSE108262 | GEO

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2018-03-15 | E-MTAB-6640 | biostudies-arrayexpress

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