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Single cell analysis of primary prostate human epithelial organoids grown in vehicle or 1,25D to day 8 and day 14

ABSTRACT: Primary prostate epithelial cells derived from a single patient were grown as organoids in vehicle or 10nM 1,25D (vitamin D). At day 8 and day 14 of culture, organoids were dissociated to single cells, and collected & prepped for RNA-seq using the 10x Genomics Chromium Genome Reagent kit v3 Chemistry. ~5,000 cells were collected from each sample and sequenced at a depth of ~50,000 genes per cells on a 2x150nt lane in a NovaSeq 6000. CellRanger alignment was performed to produce the RAW data files.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142489 | GEO | 2020/06/01

REPOSITORIES: GEO

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