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RNA-seq analysis of the spinal cord cells in scramble microPEP(Scr) or microPEP155(P155) treated EAE mouse model

ABSTRACT: The goals of this study are to analyze the DEGs of the spinal cord cells in scramble microPEP(Scr) or microPEP155(P155) treated EAE mouse model. Methods: mononuclear cells of the spinal cord were isolated using Percol™ gradient centrifugation, washed twice with PBS, and total RNA was extracted with RNAiso Plus (Takara Bio) and purified with magnetic oligo (dT) beads after denaturation. Purified mRNA samples were reverse transcribed into fragmented DNA samples and adenylated at the 3’ ends. Adaptors were ligated to construct a library. DNA was quantified by Qubit (Invitrogen). After cBot cluster generation, DNA samples were then sequenced by an Illumina HiSeq X Ten SBS instrument from Genergy Bio (Shanghai). Raw data were converted into Fastq format and transcript per million fragments mapped (FPKM) was calculated and log2 transformed with Cuffnorm. Differential gene transcripts were analyzed with DESeq and enriched for the GO/Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Results:Gene Ontology (GO) pathways analysis of RNA-seq data revealed that genes downregulated by P155 treatment were mainly involved in the immune respones signaling pathway.

ORGANISM(S): Mus musculus

PROVIDER: GSE142710 | GEO | 2019/12/31

REPOSITORIES: GEO

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RNA-seq analysis of the spinal cord cells in scramble microPEP(Scr) or microPEP155(P155) treated EAE mouse model

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