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Isolation and analysis of rereplicated DNA by Rerep-seq

ABSTRACT: Changes in gene copy number contribute to genomic instability, the onset and progression of cancer, developmental abnormalities, and adaptive potential. The origins of gene amplifications have remained elusive; however, one model suggests DNA rereplication could be a source of gene amplifications. The inability to determine which sequences are rereplicated and under what conditions have made it difficult to determine the validity of these models. Here we present RerepSeq, a technique that selectively fragments and enriches rereplicated DNA in preparation for next generation sequencing. RerepSeq is applicable to any species and can be utilized with low amounts of input DNA with rapid results. We validated RerepSeq by simulating DNA rereplication in yeast and human cells. Using RerepSeq, we demonstrate that rereplication induced in S. Cerevisiae by deregulated origin licensing is non-random and defined by broad domains that span multiple replication origins and topological boundaries.

ORGANISM(S): Homo sapiens Saccharomyces cerevisiae

PROVIDER: GSE143572 | GEO | 2020/01/22

REPOSITORIES: GEO

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