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Xrn1 modulates the dynamic osmostress response at a transcriptional and translational level

ABSTRACT: To study the role of the exonuclease Xrn1 in gene expression dynamics under osmotic stress conditions, we performed RNA-seq in Xrn1-depleted cells. By using an auxin-inducible degron, we were able to study immediate effects of Xrn1 depletion in gene expression dynamics. Therefore, we could overcome experimental limitations associated to stable deletion mutants.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE144440 | GEO | 2023/01/28

REPOSITORIES: GEO

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Project description:To study the role of the exonuclease Xrn1 in translational control, we performed ribosome profiling and RNA-seq in Xrn1-depleted cells. By using an auxin-inducible degron, we were able to study immediate effects of Xrn1 depletion in translational control. Therefore, we could overcome experimental limitations associated to stable deletion mutants.

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Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (3)

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Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (2)

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Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (4)

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