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Genome-wide CRISPR screening combined with ATAC-see assay

ABSTRACT: Cas9 expressing eHAP cells were transduced with human sgRNA lentivirus library (Doench, 2016 Nature biotech, Addgene, #73178). The cells were selected with 1 µg/ml of puromycin for 2 days, and then used for ATAC-see at 5 and 7 days post-transduction, respectively. Cells in the G2 phase were specifically gated to sort th 5% of the cells with the highest and lowest ATAC-see signal intensity(“high” or “low” population) . The sgRNA enrichment in each population was analysed by high-throughput sequencing and computationally compared by using the MAGeCK program (Li et al., Genome Biology 2014).

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE144450 | GEO | 2022/05/01

REPOSITORIES: GEO

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