Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PhosphoRiboTrap sequencing data of chemogenetic MCH neuron activation

ABSTRACT: We report phosphoRiboTrap experiments result which aim to explore the nature of the cell types in the Median Eminence (ME) regulated as a consequene of chemogenetic MCH neuron activation. Control and MCH-hM3Dq mice were 12 hrs-fasted and i.p. injected with CNO (3 mg/kg), Arcuate (ARC) and median eminence expat were extracted for preciptation of S6-marked ribosomes from both groups of mice. Extracted RNA from Immunoprecipitated ribosomes (IP) and total tissue (ARC+ME) from each mouse were subjected to deep mRNA sequencing. By analyzing the overlap of genes enriched in the IP/Input of MCH neuron activated mice with previously identified cell types using single cell mRNA sequencing of cells in the mediobasal hypothalamus (Campbell et al., 2017), we identified gene clusters which were activated upon MCH neuron activation.

ORGANISM(S): Mus musculus

PROVIDER: GSE146019 | GEO | 2020/05/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Chronic, Chemogenetic Stimulation of The Nucleus Accumbens Produces Lasting Effects on Binge Drinking and Ameliorates Alcohol-Related Transcriptional and Morphological Changes

Project description:We report RNA-Seq data obtained from nucleus accumbens tissue of HDID-1 mice subjected to chronic stimulation via CNO/DREADDs within a Drinking in The Dark (DID) Paradigm. Here, we found that chronically increasing NAc activity (via CNO/hM3Dq) decreased binge-like drinking, an effect lasting for at least 7 days . CNO did not alter intake in hM4Di- or GFP-expressing mice. We observed interesting and significant changes in expression of several plasticity-related genes (e.g. Hdac4 was increased with binge drinking, and ameliorated with CNO).

2019-06-21 | GSE133115 | GEO

Mouse spinal cords transplanted with human iPS cell-derived neural cells with and without chemogenetic stimulations on 14-days and 42-days after spinal cord injury.

Project description:hM3Dq-expressing human iPS cell-derived neural cells were transplanted in the injured mouse spinal cord. Thereafter, transplanted cells were selectively stimulated daily by intraperitoneal injection of CNO. The spinal cord 14-days and 42-days after the injury were eviscerated for analyses.

2021-11-23 | GSE173244 | GEO

Genes that were altered after chemogenetic activation of endogenous oxytocin in the dorsal horn

Project description:Purpose: RNAseq analysis was carried out to investigate the alterd genes by chemogenetic activation of endogenous oxytocin in the superficial layer (laminae I-II) and deep layer (laminae III-VI) of the dorsal horn. Methods: At 120 min after the s.c. administration of Saline or CNO (1 mg/kg) (n=3, each), adult male oxytocin-hM3Dq-mCherry transgenic rats were decapitated immediately without being anesthetized. Spinal cords specimens including laminae I-II and III-VI were collected separately. Results: 254 genes in the laminae I-II, and 191 genes in the laminae III-VI of the dorsal horn were significantly altered after chemogenetic activation of endogenous oxytocin.

2022-09-07 | GSE210528 | GEO

CNO stimulated hM3Dq-expressing iPS cell-derived neural cells

Project description:hM3Dq-expressing iPS cell-derived neural cells were stimulated by CNO treatment for two hours. Comparing to the vehicle-treated control, enhanced expressions of immediate early genes were observed.

2021-11-23 | GSE173243 | GEO

PhosphoRiboTrap sequencing data of chemogenetic MCH neuron activation

Project description:PhosphoRiboTrap sequencing data of chemogenetic MCH neuron activation

| PRJNA609068 | ENA

CNO stimulated hM3Dq-expressing iPS cell-derived neural cells

Project description:CNO stimulated hM3Dq-expressing iPS cell-derived neural cells

| PRJNA724871 | ENA

Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities

Project description:Here we aim to decipher the actions of Irx5 in the regulation of obesity and metabolic abnormalities. We employed a mouse model homozygous for an Irx5-knockout (Irx5KO) allele and conducted droplet based single-cell RNA sequencing (Drop-seq) in the hypothalamic arcuate-median eminence (ARC-ME), which is the main control center for sensing and integrating feeding regulatory signals.

2022-09-19 | GSE201736 | GEO

Orexin/Hypocretin Receptor 2 Signaling in MCH Neurons Regulates REM sleep and Insulin Sensitivity

Project description:Orexin/hypocretin receptor type 2 (Ox2R), which is widely expressed in the brain, receives orexin signals and modulates sleep and metabolism. Ox2R selective agonists are currently under clinical trials for narcolepsy treatment. Nevertheless, it remains unclear whether Ox2R exerts an inhibitory effect via Gi proteins in addition to an excitatory effect via Gq proteins. Here, we focused on Ox2R expression and function in MCH neurons, which have opposite roles to orexin neurons in sleep and metabolism regulation. Ox2R-expressing MCH neurons showed heterogeneity of RNA expression, and orexin B application in brain slices induced both excitatory and inhibitory responses in distinct MCH neuron populations. Ox2R inactivation in MCH neurons reduced transitions from NREM to REM sleep and impaired insulin sensitivity with hyperphagia. In conclusion, Ox2R mediates excitatory and inhibitory responses in MCH neuron subpopulations in vivo, which might regulate sleep and metabolism.

2024-11-19 | GSE281229 | GEO

Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

Project description:The median eminence (ME) of the hypothalamus is a structure that rapidly adapts to nutrient availability. We have used single-cell RNA sequencing to characterize the transcriptional profiles of cells specifically in the median eminence in the fasted and refed states. In our study, we focus on characterizing the oligodendrocyte population of the ME, and identifying how subpopulations respond to nutrient availability.

2021-06-30 | GSE133890 | GEO

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence

2017-02-06 | GSE90806 | GEO