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Profiling of 8-oxoG RNA modifications reveals strong functional network relationships in bronchial epitheilium following exposure to formaldehyde

ABSTRACT: Recent studies have highlighted the association between oxidative stress-inducing chemicals and diseases such as Alzheimer’s disease and cancer. While chemicals like formaldehyde are known causative agents of disease and overall negatively impact human health, their mechanisms of action remain unclear. Recent development of antibodies that target oxidized RNA nucleotides and advances in RNA-sequencing technology enable the isolation and identification of differentially oxidized transcripts. In this study, oxidation-specific immunoprecipitation is combined with RNA-sequencing to detect transcripts that are enriched in oxidized nucleotides following direct exposure of 1 ppm formaldehyde to human BEAS-2B lung cells using an air-liquid interface exposure system. Results from this analysis suggest a functional role of the oxidized transcripts in signaling pathways regulating cellular proliferation, migration, and programmed cell death. By combining direct cell-exposure systems, oxidized-RNA immunoprecipitation, RNA-sequencing technologies, and network analyses, detection of specifically oxidized molecular markers could be used to further characterize biological responses to external stressors and identify targets for drug development toward therapies for complex diseases.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148377 | GEO | 2020/10/13

REPOSITORIES: GEO

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