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Microarray profiling analysis of microRNAs expression in glioblastoma cells

ABSTRACT: Although growing evidence has revealed that ZEB2 was correlated with grades and outcome of glioma positively, the mechanisms underlying ZEB2/miRNAs-mediated gliomas development remain largely unknown. Here the authors revealed that ZEB2 raised in glioblastoma samples compared to the lower-grade gliomas. Besides, miR-637 increased to the most extent per the miRNA array analysis of cells with- or without ZEB2. ZEB2 captured the promoter of miR-637 directly and suppressed miR-637 transcription. In the absence of ZEB2, overexpressed miR-637 targeted WNT7A and repressed the WNT/β-Catenin signaling pathways subsequently, which inhibited the expression of Cyclin D1 and Vimentin. Importantly, the combination of β-Catenin-specific inhibitor LGK974 and temozolomide hampered cell proliferation synergistically in cells expressing miR-637. In line with the expectation, the inhibition of miR-637 counteracted sh-ZEB2-caused tumor regression in U251 xenograft models. The current study evaluated a novel mechanism that the association of ZEB2/miR-637/WNT7A contributed to regulate glioblastoma cell proliferation and migration in a β-Catenin-dependent manner. The results may provide new rationales to optimize the trial designs, which try to select the ideal glioblastoma patients to receive β-Catenin-targeted therapy and chemotherapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148779 | GEO | 2020/04/17

REPOSITORIES: GEO

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