Similar Datasets
Project description:We analyzed the transcriptome of P. aeruginosa grown for 16h in the presence or absence of 0.25 x MIC of polymyxinB, G3KL or T7
2019-10-23 | GSE139257 | GEO
Project description:RNA-Seq analysis of Pseudomonas aeruginosa response to testosterone
| PRJNA626622 | ENA
Project description:We report a next-generation sequencing of total RNA from Pseudomonas aeruginosa PAO1 grown in presence of rosmarinic acid (RA) 100mM. Data analysis in comparison with cells grown in absence of RA revealed that the plant compound RA induces a broad transcriptional response in this bacterium, quite similar to the quorum sensing response.
2018-07-01 | GSE103620 | GEO
Project description:The project aims to elucidate mechanisms driving P. aeruginosa response to sex steroids hormones. An experiment involving Co-immunoprecipitation combined with Mass spectroscopy was performed to identify P. aeruginosa proteins binding to estradiol and /testosterone.
2021-09-09 | PXD018897 | Pride
Project description:Hydroxy-L-proline (L-Hyp) mainly exists in animal collagens through post-translational modification on peptidyl proline. Utilization of L-Hyp as carbon and nitrogen sources in bacteria including P. aeruginosa requires conversion of L-Hyp to D-Hyp followed by the D-Hyp dehydrogenase pathway. However, the molecular mechanism in control of L-Hyp catabolism and transport at the genetic level was not clear. We performed a detailed transcriptome profile analysis of P. aeruginosa in response to L-Hyp and D-Hyp with an emphasis in catabolism and regulation, which revealed the presence of twelve genes in two adjacent loci that were induced by exogenous L-Hyp and D-Hyp. The first locus includes lhpABFE encoding a Hyp epimerase (LhpA) and D-Hyp dehydrogenase (LhpBEF), while the second locus covers genes for a putative ABC transporter (LhpPMNO), a protein of unknown function (LhpH), Hyp/Pro racemase (LhpK), and two enzymes in L-Hyp catabolism (LhpC and LhpG). In addition, proximal to these two loci, lhpR encodes a transcriptional regulator of the AraC family. We conducted four independent microarray experiments in the presence (experimental) of L-Hyp or D-Hyp. P. aeruginosa PAO1 was grown aerobically in minimal medium P with 300 rpm shaking at 37°C, in the presence of L-glutamate with the addition of L-Hyp or D-Hyp at 5 mM.
2015-09-22 | E-GEOD-73244 | biostudies-arrayexpress
Project description:Analysis of Pseudomonas aeruginosa PAO1 treated with 200 µM sphingomyelin. Results provide insight into the response to sphingomyelin in P. aeruginosa.
2017-02-12 | GSE83078 | GEO
Project description:P. aeruginosa was described to sense xenosiderophores in the extracellular environment, leading to a regulation of genes encoding the siderophore uptake proteins. Data presented here allowed to characterize the proteomic response of P. aeruginosa in presence of different xenosiderophores. PAO1 strain was grown under iron-starvation conditions with or without addition of xenosiderophores. Proteomic analyses were performed on bacterial lysates in biological triplicates.
2020-02-17 | PXD011950 | Pride
Project description:Anticipating the risk for infectious disease during space exploration and habitation is a critical factor to ensure safety, health and performance of the crewmembers. As a ubiquitous environmental organism that is occasionally part of the human flora, Pseudomonas aeruginosa could pose a health hazard for the immuno-compromised astronauts. In order to gain insights in the behavior of P. aeruginosa in spaceflight conditions, two spaceflight-analogue culture systems, i.e. the rotating wall vessel (RWV) and the random position machine (RPM), were used. Microarray analysis of P. aeruginosa PAO1 grown in the low shear modeled microgravity (LSMMG) environment of the RWV compared to the normal gravity control (NG), revealed a regulatory role for AlgU (RpoE). Specifically, P. aeruginosa cultured in LSMMG exhibited increased alginate production and up-regulation of AlgU-controlled transcripts, including those encoding stress-related proteins. This study also shows the involvement of Hfq in the LSMMG response, consistent with its previously identified role in the Salmonella LSMMG- and spaceflight response. Furthermore, cultivation in LSMMG increased heat- and oxidative stress resistance and caused a decrease in the culture oxygen transfer rate. Interestingly, the global transcriptional response of P. aeruginosa grown in the RPM was similar to that in NG. The possible role of differences in fluid mixing between the RWV and RPM is discussed, with the overall collective data favoring the RWV as the optimal model to study the LSMMG-response of suspended cells. This study represents a first step towards the identification of specific virulence mechanisms of P. aeruginosa activated in response to spaceflight-analogue conditions, and could direct future research regarding the risk assessment and prevention of Pseudomonas infections for the crew in flight and the general public.
2010-07-05 | GSE16970 | GEO
Project description:In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa to sublethal concentrations of NaClO was investigated. To this aim, four independent cultures of P. aeruginosa PAO1 grown in minimal medium BM2 were treated with NaClO (2 ug/ml) for 1 h at 37 C followed by RNA extraction and microarray analysis. Untreated cultures served as controls.
2018-04-30 | E-MTAB-2876 | biostudies-arrayexpress
Project description:The response of bacteria to the conditions at the site of infection is a key part of the transcriptional program that will determine the sucess of the infectious agent. To model the environment of the distal airway, we used bovine pulmonary surfactant (Survanta). P. aeruginosa transcript levels were measured in the presence or absence of Survanta in MOPS minimal medium to identify transcripts altered in response to surfactant. The most highly induced transcript in Survanta was PA5325, renamed sphA based on our findings that the gene was specifically induced by sphingosine derived from the sphingomyelin present in pulmonary surfactant. A divergently transcribed transcription factor, PA5324, was demonstrated to be critical for the sphingosine dependent induction of sphA and was therefore renamed SphR. Microarrays of the sphR mutant cells were compared to wild type to determine the likely SphR regulon. Wild type and sphR mutant cells were pre-grown in MOPS minimal media and then split and resuspended in MOPS pyruvate or MOPS pyruvate + surfactant
2013-12-10 | E-GEOD-48982 | biostudies-arrayexpress