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GO-CRISPR: a highly controlled workflow to improve discovery of gene essentiality

ABSTRACT: Genome-wide CRISPR screens aid researchers in discovering genes that function across a broad range of cellular mechanisms. Loss-of-function screens are particularly useful for identifying essential genes through loss of cell fitness measurements. However, loss-of-function screens are more challenging compared to gain-of-function screens due to the limited dynamic range of decreased sgRNA sequence detection. Here we describe Guide-Only control CRISPR (GO-CRISPR), an improved loss-of-function screening workflow, and its companion software package, Toolset for the Ranked Analysis of GO-CRISPR Screens (TRACS; https://github.com/developerpiru/TRACS). We demonstrate a typical GO-CRISPR workflow in a non-proliferative 3D spheroid model of dormant high grade serous ovarian cancer. We compare the results from our GO-CRISPR screen and demonstrate the discovery of novel pathways that were not possible using the currently established CRISPR screening method.

ORGANISM(S): unidentified plasmid Homo sapiens

PROVIDER: GSE150246 | GEO | 2023/04/01

REPOSITORIES: GEO

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