Transcriptomics

Dataset Information

0

Targeted single-cell RNA-seq identifies minority cell types of kidney distal nephron & A Comprehensive Map of mRNAs and Their Isoforms across All 14 Renal Tubule Segments of Mouse


ABSTRACT: Characterization of gene expression for each renal cell type is essential for identifying mechanisms underlying kidney physiology and pathophysiology. Here, we built a transcriptome landscape mouse renal epithelial cells by deep sequencing of all 14 mouse renal segments, upon which we developed an enrichment protocol to characterize distal nephron cells by single-cell RNA-seq. Our renal tubule expression atlas provided landscapes of gene expression and alternative splicing along the renal tubule segments. We identified, and integrated segment-specific genes with human gene nomenclature to understand the gene function and conservation. We further developed an enrichment strategy for single-cell analysis of distal nephron cells. The analysis revealed heterogeneous gene expression in DCT1-DCT2-CNT. Moreover, we identified two mosaic cell types in thick ascending limb and verified their presence in mouse kidney tissue. Our data provides a rich resource for kidney research and we provide our data user-friendly at https://esbl.nhlbi.nih.gov/MRECA/Nephron/ and https://esbl.nhlbi.nih.gov/MRECA/DCT/.

ORGANISM(S): Mus musculus

PROVIDER: GSE150338 | GEO | 2020/11/18

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2009-10-30 | E-GEOD-17739 | biostudies-arrayexpress
2009-10-22 | GSE17739 | GEO
2010-11-11 | E-GEOD-25223 | biostudies-arrayexpress
2022-08-25 | GSE184928 | GEO
2010-11-11 | GSE25223 | GEO
2013-11-19 | E-GEOD-51935 | biostudies-arrayexpress
2022-07-07 | MSV000089838 | MassIVE
2023-07-23 | GSE196785 | GEO
| phs001720 | dbGaP
2013-11-19 | GSE51935 | GEO