The SaeR/S Gene Regulatory System is Essential for Innate Immune Evasion by Staphylococcus aureus
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ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. In this study, we demonstrate saeR/S plays a distinct role in S. aureus pathogenesis and is vital for virulence of MW2 in a mouse model of sepsis. Moreover, deletion of saeR/S significantly impaired survival of MW2 in human blood and after neutrophil phagocytosis. Microarray analysis of genes influenced by saeR/S demonstrated SaeR/S of MW2 influences a wide variety of genes with diverse biological functions. These data shed new insight into how virulence is regulated in S. aureus and associates a specific staphylococcal gene-regulatory system with invasive staphylococcal disease.
ORGANISM(S): Rickettsia rickettsii Staphylococcus epidermidis RP62A Coxiella burnetii RSA 493 Granulibacter bethesdensis Staphylococcus epidermidis ATCC 12228 Chlamydia muridarum Chlamydia trachomatis D/UW-3/CX Staphylococcus haemolyticus JCSC1435 Borreliella burgdorferi B31 Staphylococcus aureus Chlamydia pneumoniae AR39 Staphylococcus aureus subsp. aureus MW2 Coxiella burnetii Chlamydia caviae GPIC
PROVIDER: GSE15067 | GEO | 2010/02/23
SECONDARY ACCESSION(S): PRJNA114843
REPOSITORIES: GEO
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