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Differential expression of genes associated with inflammation in KC (Kupffer cells) and MNCs (blood monocytes):

ABSTRACT: As a rule, mammalian organs contain a mixed population of macrophages from different sources of origin. It is assumed that the self-sustaining resident macrophages and the monocyte-derived transient macrophages differ by their functional properties and their roles in repair processes . The analysis of the functional features of different populations of macrophages is of significant practical importance, since macrophages are considered as therapeutic targets in various pathological conditions and as prospective basis for cell therapy products. The aim of this study was to compare gene expression profiles of the two alternative macrophage species exemplified, respectively, by Kupffer cells of the liver and monocytes of the blood.

ORGANISM(S): Mus musculus

PROVIDER: GSE150878 | GEO | 2020/05/20

REPOSITORIES: GEO

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microRNA profiles in KC (Kupffer cells) and MNCs (blood monocytes):

Project description:As a rule, mammalian organs contain a mixed population of macrophages from different sources of origin. It is assumed that the self-sustaining resident macrophages and the monocyte-derived transient macrophages differ by their functional properties and their roles in repair processes . The analysis of the functional features of different populations of macrophages is of significant practical importance, since macrophages are considered as therapeutic targets in various pathological conditions and as prospective basis for cell therapy products. The aim of this study was to compare microRNA profiles of the two alternative macrophage species exemplified, respectively, by Kupffer cells of the liver and monocytes of the blood.

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microRNA profiles in KC (Kupffer cells) and MNCs (blood monocytes):

Project description:microRNA profiles in KC (Kupffer cells) and MNCs (blood monocytes):

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COMPARATIVE ANALYSIS OF THE PROTEOME OF KUPFFER CELLS AND MONOCYTES

Project description:Macrophage population in most mammalian organs consists of cells of different origin, with the exception of the central nervous system and the liver, where macrophages of monocytic origin are almost completely absent. The reasons for such distribution and the phenomenon of coexistence of the two separate macrophage lineages with different origin in mammals remain poorly understood. In present study we compared Kupffer cells and monocytes by the immunophenotype, gene expression profile, proteome and pool of mircoRNA. Observed differences do not allow to consider the resident liver macrophages as purely M2 macrophages or monocytes have purely M1 features. Two populations of macrophages of monocytic origin and resident macrophages have fundamentally different roles in maintaining homeostasis. Monocytic macrophages are involved in the regulation of inflammation, and resident macrophages are involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis). However, if other indicators would be considered as markers of macrophage activity, it is worth noting that Kupffer cells possess some features of M2 macrophages. This is indicated by their expression profile of let-7b/c/d/e miRNAs, a high content of proteins associated with oxidative phosphorylation, as well as an increased level of synthesis of Arg1, IL10.

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Activation of GPR3-b-Arrestin2-PKM2 by DPI enhanced glycolysis in kupffer cells [Human KC]

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Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells

Project description:Self-renewing tissue-resident macrophages are thought to be exclusively derived from embryonic progenitors. However, whether circulating monocytes can also give rise to such macrophages has not been formally investigated. Here we use a new model of diphtheria toxin-mediated depletion of liver-resident Kupffer cells to generate niche availability and show that circulating monocytes engrafted in the liver, gradually adopt the transcriptional profile of their depleted counterparts and become long-lived self-renewing cells. Underlining the physiological relevance of our findings, circulating monocytes also contribute to the expanding pool of macrophages in the liver shortly after birth, when macrophage niches become available during normal organ growth. Thus, like embryonic precursors, monocytes can and do give rise to self-renewing tissue-resident macrophages if the niche is available to them. Clec4F+ Kupffer cells were isolated and sorted from livers from adult WT mice or KC-DTR or KC-DTR littermate control mice +/- 50ng DT at indicated timepoints. 19 samples (arrays) in total. RNA was isolated, amplified with Nugene pico kit, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 1.0 ST Arrays.

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The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages

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Project description:Differential expression of genes associated with inflammation in KC (Kupffer cells) and MNCs (blood monocytes):

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A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [hiPSC-EMP-KC-pKC]

Project description:To incorporate Kupffer cells into hiPSC-LOs, we differentiated erythro-myeloid progenitors (EMPs) from hiPSCs. We compares the gene expression profiles of hiPSC-EMPs with cord-blood hematopoietic stem and progenitor cells (CB-HSPCs); and EMP-generated Kupffer cells (EMP-KC) with human primary Kupffer cells.

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The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages

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