Similar Datasets
Project description:Comparison of gene expression in Maize tissues at different dev stages.
| PRJNA635889 | ENA
Project description:The genes specifically express in some tissue may usually play an important role in the development of the tissue. At vegetative growth stage, some genes may up-regulated to promote the nutrition absorbtion and transportation. However during the reproductive stage, some genes may up-regulated to regulate the flowering and seed development. We used microarrays to identify significantly differentially expressed genes between seed and non-seed tissues. Different tissues of maize were selected at vegetative growth stage and reproductive stage for RNA extraction and hybridization on Affymetrix microarrays. We sought to identify significantly differentially expressed genes between seed and non-seed tissues. To that end, Big trumpet period maize inbred line B73 was use to collect nutritive tissues include root, stem, stem tip and leaf; 10, 15, 20 and 25 DAP (days after pollination) embryo and endosperm; ear and silk before pollination. Each sample has three replications.
2014-01-23 | E-GEOD-54310 | biostudies-arrayexpress
Project description:Band9 maize B73 leaf, Bundle sheath strand section1(0-1.5cm) biological replica1 SDS-PAGE trypsin Orbitrap
2011-04-15 | PRD000190 | Pride
Project description:Epigenetic marks such as DNA methylation can act as heritable marks on a genome leading to unique regulation of genomic sequences. As a transient mark, DNA methylation has been identified as a possible mechanism for reversible genetic regulation of cells derived through either mitotic or meiotic cellular division. Although variation between epigenetic states is known to exist between individuals, there is little known about the variability of DNA methylation patterns between different developmental stages of an individual. We have assessed genome-wide DNA methylation patterns in four tissues of two inbred maize lines: B73 and Mo17. Although hundreds of regions of differential methylation are present between the two genotypes, few examples of tissue-specific DNA methylation variation were observed. The lack of clear epigenetic variation between tissues indicates the limited impact of DNA methylation on developmental processes within maize. meDIP-chip analysis of four maize tissues identifed few tissue-specific DNA methylation variable regions (tDMRs), whereas hundreds of genotype-specific DMRs were identified that were conserved across tissues.
2012-08-27 | GSE38503 | GEO
Project description:Epigenetic marks such as DNA methylation can act as heritable marks on a genome leading to unique regulation of genomic sequences. As a transient mark, DNA methylation has been identified as a possible mechanism for reversible genetic regulation of cells derived through either mitotic or meiotic cellular division. Although variation between epigenetic states is known to exist between individuals, there is little known about the variability of DNA methylation patterns between different developmental stages of an individual. We have assessed genome-wide DNA methylation patterns in four tissues of two inbred maize lines: B73 and Mo17. Although hundreds of regions of differential methylation are present between the two genotypes, few examples of tissue-specific DNA methylation variation were observed. The lack of clear epigenetic variation between tissues indicates the limited impact of DNA methylation on developmental processes within maize. meDIP-chip analysis of four maize tissues identifed few tissue-specific DNA methylation variable regions (tDMRs), whereas hundreds of genotype-specific DMRs were identified that were conserved across tissues. Methylation profiles for tassel, embryo, endosperm, and leaf of the maize inbred lines B73 and Mo17. Three biological replications for each tissue of each genotype were performed. A custom 2.1M NimbleGen array (GPL13499) was used for embryo, endosperm, and leaf, and a custom 3x1.4M NimbleGen array containing a subset of probes from the 2.1M NimbleGen array (GPL15621) was used for tassel. All of the processed data is based on the largest number of comparable probes (~1.4M) between the two arrays.
2012-08-27 | E-GEOD-38503 | biostudies-arrayexpress
Project description:RNA-seq from whole V3 maize seedlings (roots and shoots) for the B73 inbred line and A188 x B73 hybrid.
2022-01-27 | GSE175914 | GEO
Project description:The contamination of agricultural soil by heavy metal cadmium (Cd) poses a significant environmental challenge, affecting crop growth, development and, human health. Previous studies have established the pivotal role of the ZmHMA3 gene, a P-type ATPase heavy metal transporter, in determining variable Cd accumulation in maize grains among 513 inbred lines. To decipher the molecular mechanism underlying mutation-induced phenotypic differences mediated by ZmHMA3, we conducted a quantitative Tandem Mass Tag (TMT)-based proteomic analysis of immature maize kernels. This analysis aimed to identify differentially expressed proteins (DEPs) in wild-type B73 and zmhma3 null mutant under Cd stress. The findings demonstrated that zhma3 accumulated higher levels of Cd compared to B73 when exposed to varying Cd concentrations in the soil. In comparison to low Cd concentration soil, B73 and zmhma3 exhibited 75 and 142 DEPs, respectively, with 24 common DEPs shared between them. Zmhma3 showed a higher induction of upregulated genes related to Cd stres than B73. Amino sugar and nucleotide sugar metabolism were specifically enriched in B73, while phenylpropanoid biosynthesis, nitrogen metabolism, and glyoxylate and dicarboxylate metabolism appeared to play a more significant role in zmhma3. This study provides proteomics insights into unraveling the molecular mechanism underlying the differences in Cd accumulation in maize kernel.
2024-01-26 | PXD047578 | Pride
Project description:Genome-wide mapping of the TSS in root and shoot from two maize lines B73 and Mo17 Genome-wide locations and dynamics of maize core promoters obtained from the experimental establishment of the TSSs coordinates. The work derived from this data it is the first genome-wide atlas of core promoters and its dynamic generated for an important crop species. Four samples each one with biological replicates. Comparisons were done between B73 and Mo17 for each of the tissues and between tissues for each line
2015-11-04 | E-GEOD-70251 | biostudies-arrayexpress
Project description:We assembled a complete transcriptomic landscape integrating both linear and circRNAs across 26 different tissues and stages in a classic maize hybrid triplet - B73, Mo17, and their hybrid F1.
2024-03-30 | GSE199881 | GEO
Project description:Investigation of whole genome gene expression level changes in maize plants (standard maize line B73) in controlled conditions under continuous light. Tissues of the leaf elongation zone were sampled from plants well watered every 12 hours before and after lights on.
2014-03-04 | GSE55524 | GEO