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A cohesin cancer mutation reveals a role for the hinge domain in genome organization and gene expression [ChIP-seq]

ABSTRACT: Purpose: Chromatin loop formation and maintenance is regulated by the cohesin complex. Here, we investigate whether a cancer mutation to a cohesin complex subunit, SMC1A-R586W, alters genome architecture and gene expression. Method: Genome wide binding patterns of known architectural proteins and histone PTMs were assessed in WT and SMC1A-R586W mESCs by ChIP-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE153573 | GEO | 2021/02/23

REPOSITORIES: GEO

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A cohesin cancer mutation reveals a role for the hinge domain in genome organization and gene expression [Hi-C]

Project description:Purpose: Chromatin loop formation and maintenance is regulated by the cohesin complex. Here, we investigate whether a cancer mutation to a cohesin complex subunit, SMC1A-R586W, alters genome architecture. Method: Genome wide chromatin looping patterns were assessed via Hi-C in both wildtype and CRISPR-Cas9 genome edited SMC1A-R586W mouse embryonic stem cells (mESCs).

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Project description:Purpose: Chromatin loop formation and maintenance is regulated by the cohesin complex. Here, we investigate whether a cancer mutation to a cohesin complex subunit, SMC1A-R586W, alters genome architecture and gene expression. Method: Global gene expression was assessed using RNA-seq in wildtype and CRISPR-Cas9 genome edited SMC1A-R586W mouse embryonic stem cells (mESCs).

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