Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Loss of PRC2 subunits primes lineage choice during exit of pluripotency

ABSTRACT: Mouse embryoid body differentiation with single-cell analyses of timepoints 0, 4, 7 and 10. Bulk-RNA seq of directed differentiation of mouse embryonic stem cells from day 0, 5hr, 24hr and 48hrs.

ORGANISM(S): Mus musculus

PROVIDER: GSE154572 | GEO | 2020/07/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Loss of PRC2 subunits primes lineage choice during exit of pluripotency.

Project description:Polycomb Repressive Complex 2 (PRC2) is crucial for the coordinated expression of genes during early embryonic development, catalyzing histone H3 lysine 27 trimethylation. Two distinct PRC2 complexes, PRC2.1 and PRC2.2, contain respectively MTF2 and JARID2 in embryonic stem cells (ESCs). In this study, we explored their roles in lineage specification and commitment, using single-cell transcriptomics and mouse embryoid bodies derived from Mtf2 and Jarid2 null ESCs. We observe that the loss of Mtf2 results in enhanced and faster differentiation towards cell fates from all germ layers, while the Jarid2 null cells are predominantly directed towards early differentiating precursors, with reduced efficiency towards mesendodermal lineages. These effects are caused by derepression of developmental regulators that are poised for activation in pluripotent cells and gain H3K4me3 at their promoters in the absence of PRC2 repression. Upon lineage commitment, the differentiation trajectories are relatively similar to those of wild-type cells. Together, our results uncover a major role for MTF2-containing PRC2.1 in balancing poised lineage-specific gene activation, whereas the contribution of JARID2-containing PRC2 is more selective in nature compared to MTF2. These data explain how PRC2 imposes thresholds for lineage choice during the exit of pluripotency.

| S-EPMC8632979 | biostudies-literature

Loss of PRC2 subunits primes lineage choice during exit of pluripotency

Project description:Loss of PRC2 subunits primes lineage choice during exit of pluripotency

| PRJNA646712 | ENA

Effect of pioglitazone treatment on gene expression in NCI-H2347 lung cancer cells: time course experiment

Project description:Microarray studies was performed to analyze gene expression changes in NCI-H2347 cells after treatment with 50 µM pioglitazone for 12hr, 24hr and 48hrs.

2014-09-25 | GSE59735 | GEO

Effect of pioglitazone treatment on gene expression in NCI-H2347 lung cancer cells: time course experiment

Project description:Microarray studies was performed to analyze gene expression changes in NCI-H2347 cells after treatment with 50 ÂµM pioglitazone for 12hr, 24hr and 48hrs. RNA samples, containing 3 replicates for each sample, were extracted from NCI-H2347 cells after treatment with pioglitazone or vhicle (DMSO) for 12hr, 24hr and 48hrs. After biotin labeling, samples were hybridized on the Illumina Human HT12v4.0 Expression Beadchip.

2014-09-25 | E-GEOD-59735 | biostudies-arrayexpress

Expression data from undifferentiated human ES cell line, khES3, grown using complete or methionine deprived media and ES cell derived endoderm

Project description:In undifferentiated human ES cells, 5hr Met deprivation (delta Met) led to decreased proliferation, and prolonged 24hr Met deprivation resulted in G0-G1 phase cell cycle arrest, which then led to apoptosis. We used microarrays to detail the molecular mechanism of cell-death caused by Met deprivation in undifferentiated ES cells and checked the difference of Met cycle relate gene expression between undifferentiated human ES cells and ES cell derived endoderm.

2014-04-17 | GSE55283 | GEO

Comparison of pain in patient under going major surgery under general anaesthesia vs general and Epidural anaesthesia

Project description:Intervention1: General anaesthesia and epidural anesthesia vs only general anaesthesia and periopertive analgesia: 0-3-6-12-24hr Dose of ropivacaine 0.3/cc @4ml/hr acc to pt profile dose will be increased Control Intervention1: General anaesthesia only and periopertive analgesia: 0-3-6-12-24hr Primary outcome(s): Vas score assessment and need for inhalation agent perioperative periodTimepoint: 0-3-6-12-24hr Study Design: Randomized, Parallel Group Trial Method of generating randomization sequence:Computer generated randomization Method of allocation concealment:Sequentially numbered, sealed, opaque envelopes Blinding and masking:Participant and Outcome Assessor Blinded

| 2715133 | ecrin-mdr-crc

Mus musculus

Project description:CP 5hr

| PRJNA89841 | ENA

Mus musculus

Project description:HS 5hr

| PRJNA89837 | ENA

CGR8 mouse embryonic stem cells time-course experiment 0 days old vs 3 days old , 0days old vs 10 days old

Project description:time-course experiment with embryoid bodies of CGR8 mouse embryonic stem cells ; in the whole time-series RNA from 0 days old embryoid bodies were hybridized against RNA from 3 days and 10 days old embryoid bodies Keywords = embryoid bodies Keywords = mouse Keywords = time-course Keywords = oligonucleotide array Keywords: time-course

2006-04-01 | GSE2469 | GEO

Distinct PRC2 subunits regulate maintenance and establishment of Polycomb repression during differentiation.

Project description:The Polycomb repressive complex 2 (PRC2) is an essential epigenetic regulator that deposits repressive H3K27me3. PRC2 subunits form two holocomplexes-PRC2.1 and PRC2.2-but the roles of these two PRC2 assemblies during differentiation are unclear. We employed auxin-inducible degradation to deplete PRC2.1 subunit MTF2 or PRC2.2 subunit JARID2 during differentiation of embryonic stem cells (ESCs) to neural progenitors (NPCs). Depletion of either MTF2 or JARID2 resulted in incomplete differentiation due to defects in gene regulation. Distinct sets of Polycomb target genes were derepressed in the absence of MTF2 or JARID2. MTF2-sensitive genes were marked by H3K27me3 in ESCs and remained silent during differentiation, whereas JARID2-sensitive genes were preferentially active in ESCs and became newly repressed in NPCs. Thus, MTF2 and JARID2 contribute non-redundantly to Polycomb silencing, suggesting that PRC2.1 and PRC2.2 have distinct functions in maintaining and establishing, respectively, Polycomb repression during differentiation.

| S-EPMC8217195 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data