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Identification of ADAR3-bound targets in U87 cells

ABSTRACT: We report transcripts bound by ADAR3 through RNA immunoprecipitation of U87 cells expressing 3X-FLAG tagged ADAR3 compard to control cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE154862 | GEO | 2020/07/22

REPOSITORIES: GEO

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Identification of ADAR3-bound targets in U87 cells

Project description:We report transcripts bound by ADAR3 through RNA immunoprecipitation of U87 cells expressing 3X-FLAG tagged ADAR3 compard to control cells

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Identification of ADAR3-bound targets in U87 cells

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Identification of ADAR3-bound targets in U87 cells

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Project description:This study aims to elucidate potential functions of the editing-deficient ADAR3 enzyme in Mouse. This dataset aims to investigate the binding partners of the ADAR3 enzyme, both with and without RNAse treatment in order to differentiate between RNA dependent and independent interactions. The control is a Co-IP of a FLAG-tagged Luciferase protein.

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Project description:We have analyzed RNA-seq data to identify A-to-I editing sites in two groups of samples: one group isolated from human U87 cell line expressing an active ADAR3 mutant while the other isolated from U87 cell line expressing the inactive counterpart of the ADAR3 mutant. We compared these two groups of samples and identified sites whose editing levels are higher in the first group than in the second group.

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