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Identification of ADAR3-bound targets in U87 cells

ABSTRACT: Identification of ADAR3-bound targets in U87 cells

PROVIDER: PRJNA648002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12282430_1.fastq.gz	Fastqsanger.gz
	SRR12282430_2.fastq.gz	Fastqsanger.gz
	SRR12282431_1.fastq.gz	Fastqsanger.gz
	SRR12282431_2.fastq.gz	Fastqsanger.gz
	SRR12282432_1.fastq.gz	Fastqsanger.gz

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Identification of ADAR3-bound targets in U87 cells

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Project description:We have analyzed RNA-seq data to identify A-to-I editing sites in two groups of samples: one group isolated from human U87 cell line expressing an active ADAR3 mutant while the other isolated from U87 cell line expressing the inactive counterpart of the ADAR3 mutant. We compared these two groups of samples and identified sites whose editing levels are higher in the first group than in the second group.

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Project description:Although R-domain has been implicated in RNA binding in vitro, whether ADAR3 uses the arginine-rich R-domain to mediate ADAR3-RNA interactions in vivo remain to be explored. Here, an unbiased, high-throughput approach was undertaken to understand if mutating the R-domain would affect the ability of ADAR3 to either bind and/or recognize target transcripts. Although R-domain doesn’t confer target specificity to ADAR3, the R-domain mutant is associated with decreased protein stability and might reduce the ability of ADAR3 to bind target RNAs.

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