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Temporal study of the effect of acute systemic inflammation on the cerebral endothelial cells (CECs) and microglia in mice

ABSTRACT: To capture the global gene expression patterns in the CECs and microglia following acute systemic inflammation, we injected the mice with LPS and sacrificed them after 30 min, 1 hr and 2 hrs, immediately followed by the isolation of the brains and dissociation to yield single cell suspension, immunolabelling and finally flow assisted cell sorting of CECs (CD45- CD13- CD31+) and microglia (CD45 low-mid CD11b+). RNA was then isolated from the cells followed by RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE155517 | GEO | 2020/12/10

REPOSITORIES: GEO

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