ABSTRACT: RNA-Seq of HeLa cells treated with siTFIP11 or control siRNA to investigate the effect of TFIP11 knockdown on mRNA as part of a larger study on the function of TFIP11.
Project description:Small RNA-Seq of HeLa cells treated with siTFIP11 or control siRNA to investigate the effect of TFIP11 knockdown on small RNA (snRNA, snoRNA, etc.) as part of a larger study on the function of TFIP11.
Project description:RNA-Seq of HeLa cells treated with siDHX15 or control siRNA to investigate the effect of DHX15 knockdown on mRNA as part of a larger study on the function of TFIP11 and DHX15.
Project description:iCLIP of HeLa cells with anti-TFIP11 antibody to investigate RNA binding locations of TFIP11 as part of a larger study on the function of TFIP11.
Project description:RiboMethSeq of HCT116 cells treated with siTFIP11 or siDHX15 to investigate the effect of TFIP11 or DHX15 knockdown on RNA methylation as part of a larger study on the function of TFIP11.
Project description:RiboMethSeq of HCT116 cells treated with siCoilin to investigate the effect of Coilin knockdown on RNA methylation as part of a larger study on the function of the protein TFIP11.
Project description:The molecular mechanism responsible for cell fate after mitotic slippage is unclear. We investigate the postmitotic effects of different mitotic aberrations, misaligned chromosomes produced by CENP-E siRNA (siCENP-E), and monopolar spindles resulting from Eg5 siRNA (siEg5). To determine which signaling pathways contribute to the postmitotic effect of siCENP-E in the presence of siBubR1 (siCENP-E+siBubR1) compared with siEg5+siBubR1, we performed a comprehensive gene expression analysis by microarray comparisons. SUBMITTER_CITATION: Expression data of HeLa cells treated with CENP-E siRNA or Eg5 siRNA in the presence of BubR1 siRNA, Yusuke Nakayama, Akihiro Ohashi, Genomics Data, Volume 6, December 2015, Pages 44-45, doi:10.1016/j.gdata.2015.08.002
Project description:The molecular mechanism responsible for cell fate after mitotic slippage is unclear. We investigate the postmitotic effects of different mitotic aberrations, misaligned chromosomes produced by CENP-E siRNA (siCENP-E), and monopolar spindles resulting from Eg5 siRNA (siEg5). To determine which signaling pathways contribute to the postmitotic effect of siCENP-E in the presence of siBubR1 (siCENP-E+siBubR1) compared with siEg5+siBubR1, we performed a comprehensive gene expression analysis by microarray comparisons. HeLa cells were treated with siNS (non-silencing) +siBubR1, siCENP-E+siBubR1, or siEg5+siBubR1. The siNS cells were used as a control. Each siRNA treatment was duplicated. Three days after the siRNA treatments, the siRNA-transfected HeLa cells were collected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:To investigate the effect of HSATIII lncRNA on m6A modification, we performed m6A-RIP(RNA immuno precipitation) RNA-seq from heat shock-exposed HeLa cells upon HSATIII knockdown.