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IER5 is required for Notch-mediated induction of squamous cell differentiation [RNA-seq]

ABSTRACT: Conditional activation of Notch in squamous cells activates a context-specific gene expression program through lineage-specific regulatory elements. IER5 is a direct Notch-regulated gene and required for Notch-induced differentiation of squamous carcinoma cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156487 | GEO | 2020/08/31

REPOSITORIES: GEO

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IER5 is required for Notch-mediated induction of squamous cell differentiation [H3K27ac ChIP-seq]

Project description:Conditional activation of Notch in squamous cells activates a context-specific gene expression program through lineage-specific regulatory elements. IER5 is a direct Notch-regulated gene and required for Notch-induced differentiation of squamous carcinoma cells.

2020-08-31 | GSE156485 | GEO

IER5 is required for Notch-mediated induction of squamous cell differentiation [ChIP-seq RBPJ & MAML1]

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IER5 is required for Notch-mediated induction of squamous cell differentiation

Project description:IER5 is required for Notch-mediated induction of squamous cell differentiation

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IER5 is required for Notch-mediated induction of squamous cell differentiation [RNA-seq]

Project description:IER5 is required for Notch-mediated induction of squamous cell differentiation [RNA-seq]

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IER5 is required for Notch-mediated induction of squamous cell differentiation [H3K27ac ChIP-seq]

Project description:IER5 is required for Notch-mediated induction of squamous cell differentiation [H3K27ac ChIP-seq]

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IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation.

Project description:Notch signaling regulates squamous cell proliferation and differentiation and is frequently disrupted in squamous cell carcinomas, in which Notch is tumor suppressive. Here, we show that conditional activation of Notch in squamous cells activates a context-specific gene expression program through lineage-specific regulatory elements. Among direct Notch target genes are multiple DNA damage response genes, including IER5, which we show is required for Notch-induced differentiation of squamous carcinoma cells and TERT-immortalized keratinocytes. IER5 is epistatic to PPP2R2A, a gene that encodes the PP2A B55α subunit, which we show interacts with IER5 in cells and in purified systems. Thus, Notch and DNA-damage response pathways converge in squamous cells on common genes that promote differentiation, which may serve to eliminate damaged cells from the proliferative pool. We further propose that crosstalk involving Notch and PP2A enables tuning and integration of Notch signaling with other pathways that regulate squamous differentiation.

| S-EPMC7529455 | biostudies-literature

IER5 is required for Notch-mediated induction of squamous cell differentiation [ChIP-seq RBPJ & MAML1]

Project description:IER5 is required for Notch-mediated induction of squamous cell differentiation [ChIP-seq RBPJ & MAML1]

| PRJNA658109 | ENA

IER5 is required for Notch-mediated induction of squamous cell differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-08-31 | GSE156488 | GEO

IER5 is a novel Notch target gene that contributes to the effects of Notch on breast cancer stem / progenitor cells

Project description:The Notch signalling pathway has been implicated in the development and progression of numerous malignancies, including breast cancer. In breast cancer, the transcriptional programs elicited by Notch activation and the target genes responsible for mediating the downstream effects of the pathway remain poorly understood. Here, we characterise the immediate cellular consequences of Notch activation in breast cancer cells and identify a panel of direct Notch targets with potential relevance in the disease. One key direct transcriptional target of Notch in this context is IER5. Functionally, IER5 activation in breast cancer contributes to the effects of Notch signalling on the self-renewal and proliferative potential of breast cancer stem and progenitor cells. High levels of IER5 mRNA may be an important biomarker indicative of Notch activation in the disease.

2018-01-08 | GSE30288 | GEO

Notch Signaling Enhances Bone Regeneration in the Zebrafish Mandible

Project description:Loss or damage to the mandible due to trauma, treatment of oral malignancies, and other diseases is currently treated using bone grafting techniques that suffer from numerous shortcomings and contraindications. Zebrafish naturally heal large injuries to their mandibular bone, and thus offer an opportunity to understand how to boost intrinsic healing ability. Using a novel her6:mCherry Notch reporter, we show that canonical Notch signaling is induced during the initial stages of cartilage callus formation in both mesenchymal cells and chondrocytes. We also show that modulation of Notch signaling during the initial postoperative period results in lasting changes to regenerate bone quantity one month later. Notch signaling is required for mandibular bone healing, as pharmacological inhibition of Notch signaling blocks cartilage callus formation and results in non-union. Conversely, conditional transgenic activation of Notch signaling accelerates regenerative ossification. Mechanistically, we report that postoperative Notch signaling regulates multiple phases of chondroid regeneration and patterns callus metabolic landscape. Given conserved functions of Notch signaling in bone repair across vertebrates, we propose that targeted activation of Notch signaling during the early phases of bone healing may have therapeutic value.

2022-03-07 | PXD027280 | Pride

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