Transcriptomics

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Taz protects aged hematopoietic stem cells from exhaustion [CITE-seq]


ABSTRACT: We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. During the staining procedure for flow cytometry, the antibody cocktail also included antibodies labelled with oligos (antibody-derived tags =ADTs) which can later be analyzed by the 10x Chromium controller.

ORGANISM(S): Mus musculus

PROVIDER: GSE157458 | GEO | 2022/07/14

REPOSITORIES: GEO

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