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Taz protects aged hematopoietic stem cells from exhaustion [ATAC-seq]

ABSTRACT: We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. Where indicated, LT-HSCs were further fractionated into a Clca3a1 high or low population using a monoclonal antibody (10.1.1) purchased from the Developmental Studies Hybridoma Bank - DSHB.

ORGANISM(S): Mus musculus

PROVIDER: GSE157459 | GEO | 2022/07/14

REPOSITORIES: GEO

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