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Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

ABSTRACT: Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE15851 | GEO | 2010/06/25

SECONDARY ACCESSION(S): PRJNA123055

REPOSITORIES: GEO

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Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

Project description:Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control. The expression of miR-310 cluster knockout line (imprecise deletion of P{EP}2587 insertion) were compared with the control (precise deletion of P{EP}2587 insertion) using Drosophila Tiling 1.0 F array. Each stock has three biological repeats. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15851 | biostudies-arrayexpress

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome. We compared the transcriptional profiling of Dm310s and Dp310s overexpression in D.melanogaster using double-stranded cDNA followed by bioprime random labeling, and hybridization to Affy Drosophila tiling 1.0 F array. Third-instar progeny larvae from the crosses with maternal NP5941 and paternal UAS-miR-310 cluster transgenic lines were collected and third-instar larvae from the cross with maternal NP5941 and paternal w1118 were used as control. Three biological repeats were used for each stock. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15850 | biostudies-arrayexpress

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

2010-06-25 | GSE15847 | GEO

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

2010-06-25 | GSE15850 | GEO

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

2010-06-25 | E-GEOD-15847 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

| PRJNA123055 | ENA

Mouse Articular chondrocyte LC-MSMS of wild-type, miR-26a -/- and cartilage-specific miR-23ab clusters knockout mice

Project description:To reveal the potential regulation target genes of miR-26a and miR-23a/b clusters in articular chondrocytes, we performed a multi-omics analysis of LC-MSMS and RNA-seq using cultured chondrocytes samples, which were primarily isolated from 3-week-old wild-type, miR-26a -/- (with or without miR-26a mimic transfection afterwards) or miR-23a/b cluster flox/flox;Col2a1-cre mice. For LC-MSMS, protein from TRIZOL reagent was extracted, nanoLC-MSMS was performed. An expression list was made to further explore the regulation targets of miR-26a and miR-23a/b clusters.

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miR-302-367 regulates cardiomycyte proliferation and differentiation during development

Project description:To define the role of miR-302-367 cluster in cardiac development, we overexpressed miR-302-367 cluster in mouse heart by using R26R-miR-302-367; Nkx2.5-Cre mice. This data set contains the microarrays examining gene expression in the hearts of R26R-miR-302-367; Nkx2.5-Cre mice at postnatal day 14. We overexpressed miR-302-367 cluster in developing mouse heart using Nkx2.5-Cre mouse line

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Global changes in gene expression in response to miR-200 overexpression in 4TO7 cells

Project description:Transcriptional profiling of 4TO7 cells stably overexpressing miR-200s or CDH1. We observe a dramatic shift in the gene signatures when miR-200s (cluster 2; miR-200c/141, or both clusters) are overexpressed relative to controls. However, cluster 1 overexpression (miRs-200b/a/429) alone or CDH1 overexpression does not induce global changes in gene expression to levels observed for cluster 2 (miR-200c/141) or both clusters together. One cell line (4TO7) cells: four different overexpression experiments, Cluster 1, Cluster 2, Clusters 1+2, CDH1, and respective controls, Puro vec, Hygro vec, Puro+Hygro vec and Puro vec. Each experiment has two biological replicates. Total, 16 samples.

2010-12-23 | E-GEOD-19631 | biostudies-arrayexpress

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