Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome. We compared the transcriptional profiling of Dm310s and Dp310s overexpression in D.melanogaster using double-stranded cDNA followed by bioprime random labeling, and hybridization to Affy Drosophila tiling 1.0 F array. Third-instar progeny larvae from the crosses with maternal NP5941 and paternal UAS-miR-310 cluster transgenic lines were collected and third-instar larvae from the cross with maternal NP5941 and paternal w1118 were used as control. Three biological repeats were used for each stock. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15850 | biostudies-arrayexpress

Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

2010-06-25 | GSE15851 | GEO

Expression profiling of dme-miR-310 cluster (miR-310, miR-311, miR-312, miR-313) knockout

Project description:Expression profiling of the dme-miR-310 cluster knockout line in D.melanogaster. The knockout line was generated in an imprecise P-element excision screen using a stock P{EP}2587 containing a P-element insertion directly upstream of the miR-310 cluster. The precise excision line of P{EP}2587 was used as the control. The expression of miR-310 cluster knockout line (imprecise deletion of P{EP}2587 insertion) were compared with the control (precise deletion of P{EP}2587 insertion) using Drosophila Tiling 1.0 F array. Each stock has three biological repeats. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15851 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

| PRJNA123053 | ENA

Drosophila melanogaster

Project description:Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

| PRJNA123049 | ENA

Drosophila melanogaster

Project description:8-miR cluster deletion affects mef2 levels in Drosophila melanogaster

| PRJNA143007 | ENA

Trans regulatory evolution of a young microRNA during Drosophila spermatogenesis

Project description:Both cis and trans regulatory evolution underlies phenotypic innovation and diversification. However, how the nascent trans regulators escape negative pleiotropy, which may constrain their evolution and contribution to adaptation, remain unclear. Here, we compare the testis transcriptomes at single-cyst level between knockout lines of miRNA-983, an adaptively evolving young miRNA, in D. melanogaster and D. simulans, and the replacement line of dme-miR-983 with dsi-miR-983 in D. melanogaster. By distinguishing context effects and miR-983 effects in the framework of cis and trans regulation of gene expression, we demonstrate that miR-983 contributes to interspecific expression divergence in spite of its antagonism with context effects which is often predominant. Gain and loss of miR-983 effects involve distinct gene sets featured in different phases of spermatogenesis, which may help minimize pleiotropy. The magnitude of miR-983 effects is negatively associated with the variability of target gene expression dynamics during spermatogenesis. Moreover, knocking out dme-miR-983 in D. melanogaster affects sperm length and sperm competitive ability, which can be rescued partially by dsi-miR-983. Our results provide empirical evidence for the resolution of negative pleiotropy, and also have broad evolutionary implications for the biological function of trans regulators.

2022-07-13 | GSE190885 | GEO

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