Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of corticosterone treatment on the transcriptome of mouse brain endothelial bEnd3 cells

ABSTRACT: We performed transcriptome profiling of bEnd3 cells treated in vitro with 5uM corticosterone or medium for 24 hours

ORGANISM(S): Mus musculus

PROVIDER: GSE159756 | GEO | 2021/03/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The effect of corticosterone treatment on the proteome of mouse brain endothelial bEnd.3 cells

Project description:Elevated glucocorticoids feature body’s responses to a variety of psychological and physiological stressors. To understand how glucocorticoids affect brain endothelial cells that form the blood-brain barrier, we applied quantitative proteomics to study changes in the proteome of a mouse brain endothelial cell line, bEnd3, upon acute 24-hour treatment with 5 uM corticosterone in vitro.

2021-03-01 | PXD022166 | Pride

The effect of corticosterone treatment on the transcriptome of mouse brain endothelial bEnd3 cells

Project description:The effect of corticosterone treatment on the transcriptome of mouse brain endothelial bEnd3 cells

| PRJNA670426 | ENA

Role of BEND3 in transcription repression

Project description:We have uncovered that BEND3 is a heterochromatin-associated protein that functions as a transcription repressor. Expression of BEND3 correlates with the state of pluripotency, with a significant decline in BEND3 levels in differentiated cells. Cells lacking BEND3 show defects in cell cycle progression with failure to enter S-phase. BEND3 affects the transcription of cellular genes that modulate chromatin organization and cellular differentiation and that downregulation of BEND3 is critical for cellular differentiation.

2022-02-08 | GSE151235 | GEO

BEND3 safeguards pluripotency by repressing differentiation-associated genes

2022-02-08 | GSE171091 | GEO

Effect of corticosterone treatment on rat neuronal progenitor cells

Project description:Exposure to excessive glucocorticoids, which are major mediators of the stress reaction, is detrimental to brain development related to an increased risk of psychiatric disorders. However, the molecular mechanism underlying the effect of excessive glucocorticoids on the developing brain remains largely unclear. To clarify the mechanism, we examined the effects of glucocorticoids on cultured neuronal progenitor cells (NPCs) from cerebral cortices from rat embryo. We performed comparative analysis using gene expression profiling of corticosterone- or vehicle-treated NPCs. Results provide insight into the effects of glucocorticoids in regulating genetic programs important for controlling NPCs’ properties. Keywords: expression analysis, corticosterone, neuronal progenitor, cortex

2009-03-31 | GSE11478 | GEO

Expression data from Smad4-siRNA bEnd3 cells

Project description:TGF-beta/Smads signaling plays important roles in vascular integrity. To identify potential Smad4 target genes in brain endothelial cells that control cerebrovascular integrity, the microarray assay was performed to compare the gene expression profiles of bEnd3 transfected with Smad4-siRNA and control-siRNA. bEnd3 cells were infected by Smad4-siRNA or control-siRNA retrovirus particles produced by PLAT-E packaging cells and selected by puromycin. The specific and control RNAi cells were used for RNA extraction and hybridization on Affymetrix microarrays. Two independent infections were performed and samples were pooled in order to obtain representative populations.

2011-04-17 | E-GEOD-15736 | biostudies-arrayexpress

Transcription profiling of rat brain cortex from corticosterone treated animals

Project description:Animal models represent a very useful tool for the study of depressive-like behaviour and for the evaluation of the therapeutic efficacy of antidepressants. Nevertheless, gene expression patterns of these different animal models and whether genes classically associated to human major depression are present in these genetic profiles remain unknown. Gene expression was evaluated in an animal model of depression: corticosterone-treated rats. Gene expression analyses were carried out using the AffymetrixÂ® GeneChipÂ® technology. Experiment Overall Design: In total, 19 animals were studied, 10 corticosterone-treated rats and 9 control rats

2008-06-16 | E-GEOD-9797 | biostudies-arrayexpress

Expression data from Smad4-siRNA bEnd3 cells

2011-04-17 | GSE15736 | GEO

Adrenalectomy plus corticosterone treatment, rat hippocampal RNA-seq

Project description:Male Sprague-Dawley rats 8 weeks old, were adrenalectomized, treated with 300ug/kg corticosterone or vehicle 3 days after surgery then sacrificed 1 hour later. Hippocampi were removed and RNA extracted and processed for sequencing at the Massachusetts General Hospital Nex-Generation Sequening Core. Includes 6 cort treated and 6 control biological replicates

2016-08-11 | E-GEOD-84249 | biostudies-arrayexpress

A genetic screen identifies BEND3 as a regulator of bivalent gene expression and global DNA methylation

Project description:Epigenetic mechanisms are essential to establish and safeguard cellular identities in mammals. They dynamically regulate the expression of genes, transposable elements and higher-order chromatin structures. Consequently, these chromatin marks are indispensable for mammalian development and alterations often lead to disease, such as cancer. Bivalent promoters are especially important during differentiation and development. Here we used a genetic screen to identify new regulators of a bivalent repressed gene. We identify BEND3 as a regulator of hundreds of bivalent promoters, some of which it represses, and some of which it activates. We show that BEND3 is recruited to a CpG-containg consensus site that is present in multiple copies in many bivalent promoters. Besides having direct effect on the promoters it binds, the loss of BEND3 leads to genome-wide gains of DNA methylation, which are especially marked at regions normally protected by the TET enzymes. DNA hydroxymethylation is reduced in Bend3 mutant cells, possibly as consequence of altered gene expression leading to diminished alpha-ketoglutarate production, thus lowering TET activity. Our results clarify the direct and indirect roles of an important chromatin regulator, BEND3, and, more broadly, they shed light on the regulation of bivalent promoters.

2023-08-26 | GSE224077 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data