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Single cell- Cluster-RNA seq and Spatial Transcriptomics from the trunk of mouse embryos of different genotypes (wild type, Pax2-GFP, Pax2-GFP; Gata3 knockout) and developmental stages (E8.75, E9.5, E11.5) [single-cell RNA-seq]

ABSTRACT: To study cell heterogeneity and state transitions in the developing urinary tract we isolated renal cells (single cells or clusters) by Fluorescence-activated cell sorting (FACS) from the trunk of E8.75, E9.5 and E11.5 mouse embros and performed 10X Genomics single cell and Cluster RNA sequencing

ORGANISM(S): Mus musculus

PROVIDER: GSE160136 | GEO | 2021/02/16

REPOSITORIES: GEO

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Single cell- and Clumps-RNA seq from the trunk of E9.5 Pax2-GFP and Pax2-GFP; Gata3 knockout embryos

Project description:To study cell heterogeneity and state transitions in the developing urinary tract we isolated Pax2-GFP positive single cells or clumps of cells by Fluorescence-activated cell sorting (FACS) from the trunk of E9.5 Pax2-GFP BAC transgenic embryos and performed 10X Genomics single cell and Clumps RNA sequencing

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Single cell- and Clumps-RNA seq from the trunk of E9.5 Pax2-GFP and Pax2-GFP Gata3 knockout embryos

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Single cell- Cluster-RNA seq and Spatial Transcriptomics from the trunk of mouse embryos of different genotypes (wild type, Pax2-GFP, Pax2-GFP Gata3 knockout) and developmental stages (E8.75, E9.5, E11.5) [Spatial Transcriptomics]

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