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Single cell- Cluster-RNA seq and Spatial Transcriptomics from the trunk of mouse embryos of different genotypes (wild type, Pax2-GFP, Pax2-GFP Gata3 knockout) and developmental stages (E8.75, E9.5, E11.5) [single-cell RNA-seq]

ABSTRACT: Single cell- Cluster-RNA seq and Spatial Transcriptomics from the trunk of mouse embryos of different genotypes (wild type, Pax2-GFP, Pax2-GFP Gata3 knockout) and developmental stages (E8.75, E9.5, E11.5) [single-cell RNA-seq]

PROVIDER: PRJNA672073 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12899603_1.fastq.gz	Fastqsanger.gz
	SRR12899603_2.fastq.gz	Fastqsanger.gz
	SRR12899604_1.fastq.gz	Fastqsanger.gz
	SRR12899604_2.fastq.gz	Fastqsanger.gz
	SRR12899605_1.fastq.gz	Fastqsanger.gz

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Project description:Heterozygous and homozygous Pax2 E11.5 embryos were collected and the intermediate mesoderm was dissected and dispersed into single cells. The Pax2 positive cells also expressed EGFP, which was knocked into the Pax2 locus. EGFP positive cells were sorted by FACS and RNA isolated. We compared RNA expression levels in EGFP positive cells from Pax2 null and Pax2 heterozygote embryos.

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