Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-sequencing of adipose-derived stem cells

ABSTRACT: We performed single-cell RNA-sequencing to uncover the well-documented heterogeneity in an unbiased manner from the adipose-derived stem cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE160560 | GEO | 2023/10/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Multilayered Omics Revealsl Sex- and Depot-Dependent Adipose Progenitor Cell Heterogeneity

Project description:White adipose tissue (WAT) harbors functionally diverse subpopulations of adipose progenitor cells that differentially impact tissue plasticity in a sex- and depot-dependent manner. To date, the molecular basis of this cellular heterogeneity has not been fully defined. Here, we describe a multilayered omics approach to dissect adipose progenitor cell heterogeneity from in three dimensions: progenitor subpopulation, sex, and anatomical localization. We applied state-of-the-art mass spectrometry methods to quantify 4870 proteins in eight different stromal cell populations from perigonadal and inguinal WAT of male and female mice and acquired transcript expression levels of 15477 genes using RNA-seq. Notably, our data highlight the molecular signatures defining sex differences in PDGFR+ preadipocyte differentiation and identify regulatory pathways that functionally distinguish adipose tissue PDGFRb+ subpopulations. The data are freely accessible as a resource at "Pread Profiler. Together, the multilayered omics analysis provides unprecedented insights into adipose stromal cell heterogeneity.

2023-03-11 | PXD023829 | Pride

Human adipose-derived stem cells secretome

Project description:This project is a report of protemoic data of secretome from human adipose tissue-derived stem cells. The ADSCs were cultured in serum-free condition, and LC-MS/MS identification was conducted to analyze the ingredients in the secretome.

2022-02-17 | PXD026436 | Pride

Adipose-derived mesenchymal stem cell extracellular vesicles downregulate inflammation in osteoarthritis chondrocytes and cartilage explants

Project description:Osteoarthritis (OA) is a joint condition associated with articular cartilage loss, low-grade synovitis and alterations in subchondral bone and periarticular tissues. In OA, the interest for mesenchymal stem cell (MSC)-EV therapeutic applications has increased. We have assessed the immunomodulary properties of adipose-derived MSCs (AD-MSCs) microvesicles (MV) and exosomes (EX) in interleukin (IL)-1β stimulated OA chondrocytes and cartilage explants and characterized them by mass spectrometry in order to uncover novel mediators in (AD-MSC)-EV immunomodulation.

2022-02-23 | PXD009077 | Pride

Single-cell RNA-seq of the human Lineage– cells derived from the adipose stromal vascular fraction across depots

Project description:We used single-cell RNA-seq to characterize the heterogeneity of the human Lin- stromal vascular fraction across five adipose depots. We mollecularly assessed Lin- SVF cells from subcutaneous, omentum, mesocolic, and perirenal fat depots, as well as gallbladder-associated fat using 10x Genomics.

2023-09-30 | E-MTAB-12893 | biostudies-arrayexpress

Screening of Adipose mesenchymal stem cell derived secretome

Project description:The objective of this project is secreening the constituent of adipose derived mesenchymal stem cell secretome.

2023-09-11 | PXD045254 |

Single cell RNA sequencing of cultured endometrial mesenchymal stem cells

Project description:Endometrial mesenchymal-like stem cells (eMSCs) are adult stem cells contributing to endometrial regeneration. One set of perivascular markers (CD140b+CD146+) have been widely used to enrich eMSCs. In this study, we applied 10X genomics single-cell RNA sequencing to eMSCs cultured in vitro after microbeading from 6 donors to investigate cellular heterogeneity in an unbiased manner.

2021-06-01 | GSE149651 | GEO

Low and High Lamin A/C Adipose-Derived Stem Cells

Project description:Proteomics for sorted low vs. high 20% of lamin A/C-expressing primary, human, adipose-derived stem cells compared to an unsorted population

2021-05-28 | MSV000087538 | MassIVE

Comparison between Periprostatic adipose tissue and abdominopelvic adipose tissue

Project description:White adipose tissue accumulates at various sites throughout the body and some adipose tissue depots exist in close proximity to organs, whose function they could influence in a paracrine manner. Prostate gland is surrounded by a poorly characterized adipose depot called periprostatic adipose tissue (PPAT) playing emerging roles in prostate-related disorders. Unlike all other adipose depots, PPAT secretes pro-inflammatory cytokines even in lean individuals and does not increase in volume during obesity. These unique features remain unexplained due to the poor structural and functional characterization of this tissue. We characterized the structural organization of PPAT in patients in comparison to abdominopelvic adipose tissue (APAT), an extraperitoneal adipose depot whose accumulation is correlated to BMI. Unbiased comparisons of PPAT and APAT transcriptomes found that most differentially expressed genes were related to the hypoxia response. This chronic hypoxic state was associated with inflammation and fibrosis, which explain the failure of PPAT to expand in obesity and open new mechanistic avenues to explain its role in prostate-related disorders including cancer.

2022-03-11 | GSE196442 | GEO

Single-cell RNA-sequencing of adipose-derived stem cells

Project description:Single-cell RNA-sequencing of adipose-derived stem cells

| PRJNA673525 | ENA

RNA sequencing of inguinal white adipose tissue from seipin-deficient mice and appropriate controls

Project description:We generated Bscl2lox/lox mice X ERT2-Adipoq-CRE mice, allowing inducible seipin deletion in adipocytes upon tamoxifen addition (iATSKO). Indeed, a 5-day tamoxifen treatment led to a dramatic decrease in Bscl2 mRNA in the adipose tissue. To study the effects of seipin deficiency in vivo on adipose tissue homeostasis in an unbiased manner, we used 3’-signal recognition particle (SRP) RNA sequencing.

2022-01-11 | GSE188544 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data