Project description:Transcriptional comparison of two subpopulations of stem cells isolated from lipoaspirated human adipose tissue, profiling Adipose Stem Cells (ASCs) and Multilineage Differentiating Stress-Enduring Adipose Tissue cells (MUSE-ATs). Experiments focused in the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells, termed MUSE-ATs, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a homogenous, highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. The goal of this experiment was to characterize MUSE-ATs, as well as compare them to the endogenous subpopulation of ASCs for reference. Single condition experiment, MUSE-ATs vs. ASCs. Biological replicates: 3 MUSE-ATs replicates, 3 ASCs replicates for reference.
Project description:This project is a report of protemoic data of secretome from human adipose tissue-derived stem cells. The ADSCs were cultured in serum-free condition, and LC-MS/MS identification was conducted to analyze the ingredients in the secretome.
Project description:We performed RNA sequencing on the sorted adipose stem cells (ASCs) from multiple white adipose tissue depots to analyze the differential expression and pathway enrichment analysis of adipose stem cells from different anatomical sites.
Project description:Exosomes were extracted from adipose mesenchymal stem cells of Qnling giant pandas using ultracentrifugation. Proteomic techniques were applied to analyze the composition and function of ADMSC-EX.
Project description:Transcriptional comparison of two subpopulations of stem cells isolated from lipoaspirated human adipose tissue, profiling Adipose Stem Cells (ASCs) and Multilineage Differentiating Stress-Enduring Adipose Tissue cells (MUSE-ATs). Experiments focused in the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells, termed MUSE-ATs, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a homogenous, highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. The goal of this experiment was to characterize MUSE-ATs, as well as compare them to the endogenous subpopulation of ASCs for reference.
Project description:Dedifferentiated Fat Cells (DAs) and Adipose-derived stem cells (ADSCs) are two main stem cells derived from adipose tissue. In order to explore whether there is difference in transcriptome between the two cells after multiple subcultures, and which kind of cell should be selected for different regenerative aim, high-throughput RNAseq were conducted to find differentially expressed genes. The subcutaneous adipose tissue of three young women (23-30 years old)patients with normal BMI (19-24) were obtained during liposuction operation on abdomen or thigh. Stromal Vascular Fraction (SVF) and adipocytes were obtained by collagenase digestion, ADSCs was cultured regularly, and DAs were cultured by a modified ceiling culture method. Only 186 genes with different expression between the two groups were obtained.The downregulated gene number of DAs vs. ADSCs was 112 and the upregulated number was 74. Many of the differentially expressed genes are involved in the biological functions such as transcription regulator, protein translation regulation, cytokine interaction and energy metabolism regulation. These data may provide a foundation for further clinical administration of stem cells derived from adipose tissues.
Project description:Proteome experiment was peformed on exosomes of human minor salivary gland mesenchymal stem cells and adipose-derived stem cells to find out the same points and difference of these two kinds of exosomes, which can hopefully give further guidance on further therapy research
Project description:[1] Microarray analysis in the rat myocardial tissue: 124I-HIB transplanted MI model Vs. phosphate buffered saline (PBS) injected myocardial infarction (MI) model Vs. Sham operated model [2] Microarray analysis in the rat adipose derived stem cells: 124I-HIB-labeled ADSCs Vs. Unlabeled ADSCs