Transcriptomics

Dataset Information

0

SLAMF5 blocking of sorted Bregs CD19+IL10+ (GFP+)


ABSTRACT: Purpose: Our results show that in the absence of SLAMF5 ( by KO or blocking ab), there is an accumulation of Bregs (CD19+IL10+) and a specific increase of their survival. Here, using RNAseq, we wished to identify genes that take place in the regulation of Breg by SLAMF5 blocking. Method: Total RNA was extracted from 3x10e5 sorted CD19+GFP+cells from 2-3 Vert-x mice (induced with EAE) incubated with SLAMF5 blocking Ab or control IgG using the Direct-zol RNA kit (ZymoResearch) according to the manufacturer’s instructions. RNA-seq was performed using the MARS-seq protocol. Showing here 2 biological replicates of each group.

ORGANISM(S): Mus musculus

PROVIDER: GSE160828 | GEO | 2021/01/25

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA674605 | ENA
2024-02-20 | GSE168523 | GEO
2022-09-29 | GSE208527 | GEO
2019-10-15 | E-MTAB-7345 | biostudies-arrayexpress
2019-10-15 | E-MTAB-7375 | biostudies-arrayexpress
2019-10-15 | E-MTAB-7525 | biostudies-arrayexpress
2022-07-31 | GSE181765 | GEO
2023-09-20 | GSE242200 | GEO
2018-01-25 | E-MTAB-6381 | biostudies-arrayexpress
2021-08-03 | GSE178713 | GEO