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Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

ABSTRACT: We performed ATAC-seq in a epiblast stem cell model of mouse gastrulation to determine the effect of Mixl1 knockout and induction on chromatin accessibility.

ORGANISM(S): Mus musculus

PROVIDER: GSE161163 | GEO | 2024/07/27

REPOSITORIES: GEO

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Transcriptomic changes in mouse Epiblast stem cells induced with Mixl1

Project description:We performed total RNA-seq in a epiblast stem cell model of mouse gastrulation to determine the effect of Mixl1 knockout and induction.

2024-07-27 | GSE161165 | GEO

Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

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Single-cell RNAseq of Mixl1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development.

Project description:Chimeric embryos were generated to investigate the effect of Mixl1 knockout in mouse embryos by single-cell RNA-sequencing. Mixl1 is an essential transcription factor for mesendoderm development. Chimeric embryos contain tissue that is Mixl1+/+, which prevents global developmental failures. Embryos were generated by blastocyst injection of tdTomato-labelled, Mixl1-/- mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

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