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Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

ABSTRACT: We performed ChIP-seq in a epiblast stem cell model of mouse gastrulation to identify the genomic binding locations of mouse MIXL1

ORGANISM(S): Mus musculus

PROVIDER: GSE161164 | GEO | 2024/07/27

REPOSITORIES: GEO

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Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

Project description:We performed ATAC-seq in a epiblast stem cell model of mouse gastrulation to determine the effect of Mixl1 knockout and induction on chromatin accessibility.

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Transcriptomic changes in mouse Epiblast stem cells induced with Mixl1

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Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

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Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

Project description:Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

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Transcriptomic changes in mouse Epiblast stem cells induced with Mixl1

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Project description:Identification of Ta and Tbx16 binding sites during gastrulation, and Mixl1 binding sites during endoderm specification

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The epigenetic modifier Fam208a is required to maintain epiblast cell potency

Project description:Gastrulation initiates with the formation of the primitive streak, during which, cells of the epiblast delaminate to form the mesoderm and definitive endoderm. At this stage, the pluripotent cell population of the epiblast undergoes very rapid cellular proliferation and extensive epigenetic programming. Here, we show that Fam208a, a new epigenetic modifier, is essential for early post-implantation development using mouse strains harbouring two different allelic mutations. We show that at E6.5, Fam208a mutants have a decreased number of Oct4-positive epiblast cells due to an increase in p53-driven apoptosis. Complete removal of p53 could rescue the gastrulation block in Fam208a mutants, enabling them to develop until E8.5-9.0. The data demonstrates a new in vivo function of Fam208a in maintaining epiblast fitness thereby, making it an important factor at the onset of gastrulation.

2017-06-01 | E-MTAB-5357 | biostudies-arrayexpress

hESC MIXL1+ MIXL1- microarray

Project description:Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated â¥2-fold in the eGFP+ (MIXL1+) mesoderm population A stencil differentiation protocol was used to isolate mesodermal cells based on GFP expression from the MIXL1 locus. Microarray analysis of isolated cells from day 3 of differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes.

2015-11-05 | E-GEOD-74664 | biostudies-arrayexpress

hESC MIXL1+ MIXL1- microarray

2015-11-05 | GSE74664 | GEO

Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

Project description:The pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) and primed epiblast stem cells (EpiSCs), respectively. However, these two states of pluripotency may not be sufficient to reflect the full complexity and developmental potency of the epiblast during mammalian early development. Here we report the establishment of self-renewing formative pluripotent stem cells (fPSCs) which manifest features of epiblast cells poised for gastrulation. fPSCs can be established from different mouse ESCs, pre-/early-gastrula epiblasts and induced PSCs. Similar to pre-/early-gastrula epiblasts, fPSCs show the transcriptomic features of formative pluripotency, which are distinct from naïve ESCs and primed EpiSCs. fPSCs show the unique epigenetic states of E6.5 epiblast, including the super-bivalency of a large set of developmental genes. Just like epiblast cells immediately before gastrulation, fPSCs can efficiently differentiate into three germ layers and primordial germ cells (PGCs) in vitro. Thus, fPSCs highlight the feasibility of using PSCs to explore the development of mammalian epiblast.

2021-02-19 | GSE154290 | GEO

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