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Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

ABSTRACT: Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

PROVIDER: PRJNA675789 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13016542_1.fastq.gz	Fastqsanger.gz
	SRR13016542_2.fastq.gz	Fastqsanger.gz
	SRR13016543_1.fastq.gz	Fastqsanger.gz
	SRR13016543_2.fastq.gz	Fastqsanger.gz
	SRR13016544_1.fastq.gz	Fastqsanger.gz

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Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

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Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

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hESC MIXL1+ MIXL1- microarray

Project description:Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated â¥2-fold in the eGFP+ (MIXL1+) mesoderm population A stencil differentiation protocol was used to isolate mesodermal cells based on GFP expression from the MIXL1 locus. Microarray analysis of isolated cells from day 3 of differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes.

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Change in Expression in U937 under MIXL1 enforced overexpression

Project description:While the role of the paired-type homeobox transcription factor MIXL1 in early embryonic development has been previously established, very little is known about the role of MIXL1 in adult hematopoiesis. To determine what role MIXL1 plays in adult hematopoietic cells, we established an enforced expression model for MIXL1 in the cell line U937, then analyzed the changes of gene expression caused by increased expression of MIXL1 by microarray analysis.

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