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Time course transcriptomic analysis of human melanoma A375 cells dying of immunogenic or non immunogenic apoptosis

ABSTRACT: We set out to investigate the transcriptional profile of cancer cells responding to mitoxantrone (MTX) or hypericin-based photodynamic therapy (Hyp-PDT), as prototypes of immunogenic treatments compared to cisplatin (CDDP), considered a poorly immunogenic chemotherapeutic. We isolated the bulk-RNA of the treated cells, at 0 hr (untreated) and 4 hr (pre-apoptotic), 10 hr (early apoptotic) and 20 hr (late apoptotic) post-treatment, and compared it to their respective time-matched untreated controls. Differential gene expression analysis revealed that MTX and Hyp-PDT significantly upregulated the transcription of several genes as early as 4 hr post-treatment, whereas responses to CDDP treatment occurred mainly at the later time points and were associated with a predominant transcript downregulation. Bioinformatics analyses showed pathways relative to chemokine signaling and inflammation as significantly associated selectively to cells dying in an immunogenic fashion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163377 | GEO | 2020/12/17

REPOSITORIES: GEO

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Time course transcriptomic analysis of human melanoma A375 cells dying of immunogenic or non immunogenic apoptosis

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