Transcriptomics

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MRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration


ABSTRACT: Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench.

ORGANISM(S): Mus musculus

PROVIDER: GSE164283 | GEO | 2022/01/19

REPOSITORIES: GEO

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