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MRNA profiles of subfractions within hematopoietic stem cells in bone marrow

ABSTRACT: Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: One hundred cells of subfractions within the HSC fraction were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench.

ORGANISM(S): Mus musculus

PROVIDER: GSE187376 | GEO | 2022/01/19

REPOSITORIES: GEO

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single ATAC-seq of subfractions within hematopoietic stem cells in bone marrow

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: One hundred thousand cells of subfractions within the HSC fraction were sorted. These cells were subjected to ATAC-sequence by using 10X Genomics Chromium Next GEM Single Cell ATAC Reagent Kits v1.1. The sequence reads that passed quality filters were analyzed by FASTX-toolkit.

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Project description:Purpose: The goals of this study are to elucidate the underlying charactristic difference of p5 neonatal and 4 weeks old hematopoietic stem cell. Methods: p5 neonatal and 4 weeks old 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench.

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Subfractions within hematopoietic stem cells in bone marrow

Project description:Subfractions within hematopoietic stem cells in bone marrow

| PRJNA777564 | ENA

mRNA profiles of hematopoieitc stme cells treated with interferon gamma and/or vitronectin

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2019-07-17 | GSE134329 | GEO

Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Monocyte-Depleted Brain Transcriptomes

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