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To investigate the downstream target gene of YAP1 in enzalutamide-resistant (EnzaR) cells and the effects of extracellular vehicles (EVs) derived from EnzaR cells on enzalutamide-sensitive cells by next-generation sequencing analyses

ABSTRACT: Our study has identified that yes-associated protein 1 (YAP1) is overexpressed in enzalutamide-resistant (EnzaR) cells. Furthermore, enzalutamide-induced YAP1 expression is mediated through the function of chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII) at the transcriptional and the post-transcriptional levels. Furthermore, both YAP1 and COUP-TFII have proven to be cargoes of EVs by our study. Next, treatment with EnzaR-EVs induces the abilities of enzalutamide resistance in its parental cells while depletion of YAP1 or COUP-TFII in EnzaR-EVs attenuated it. In order to systemically investigate the downstream target gene of YAP1 and the expression profiles changed by EnzaR-EVs from control, YAP1 and COUP-TFII depletions in prostate cancer cells, we have performed RNA-seq analyses to pursuit the research directions of those questions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE164880 | GEO | 2024/01/13

REPOSITORIES: GEO

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Orphan nuclear receptor COUP-TFII enhances myofibroblasts glycolysis leading to kidney fibrosis

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Expression data from control and COUP-TFII siRNA treated PC3 cells

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Project description:Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII; NR2F2) is an orphan nuclear receptor involved in cell-fate specification, organogenesis, angiogenesis and metabolism. Ablation of COUP-TFII in the mouse uterus causes infertility due to defects in embryo attachment and impaired uterine stromal cell decidualization. Although the function of COUP-TFII in uterine decidualization has been described in mice, its role in the human uterus remains unknown. To better elucidate the mechanisms with which COUP-TFII regulates target gene transcription, genome-wide COUP-TFII binding sites in human endometrial stromal cells (HESC) treated with deciduogenic hormones were identified using ChIP-seq. A total of 16,298 intervals (binding regions) for COUP-TFII were identified compared with the input in HESC chromatin with a very low false discovery rate (0.17%) using a stringent cutoff of p =1x10-10. Distribution of intervals showed that more than half (58.6%) of the COUP-TFII binding sites are located within 10 kb of gene boundaries. 7.5% of total intervals reside within the 10 kb promoter region. A total of 6,077 unique genes were identified to have COUP-TFII binding sites within 10 kb of their gene boundaries.

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