Project description:MicroRNA transcriptional profiling of male Sprague-Dawley rats rat brains comparing microRNA abundance in five different brain regions. Two-condition experiment, regional brain samples vs. universal brain reference. Biological replicates: 4 samples from each region. One sample per array. Each array is divided into 3 replicate subarrays.
Project description:This program addresses the gene signature associated with LPS-treated liver in rat. Specifically, which genes are differentially expressed in livers of the Sprague Dawley rats treated with LPS?
Project description:Male Sprague Dawley rats were treated daily by oral gavage with either corn oil, chlorpyrifos, or PF-04457845 (selective FAAH inhibitor) from postnatal day10-16. The rats were scarified on postnatal day 38, and brains were collected and stored at -80˚C. Amygdala was isolated from the brain, homogenized the tissue, proteins were quantified, trypsin digested, and analyzed by LC ESI MS/MS.
Project description:This program addresses the gene signature associated with LPS-treated liver in rat. Specifically, which genes are differentially expressed in livers of the Sprague Dawley rats treated with LPS? The LPS-treated liver profiling data was analyzed by identifying genes that were up- and down-regulated at selected p value and fold change in livers of the Sprague Dawley rats treated with LPS compared to the corresponding non-treated controls.
Project description:MicroRNA profiling of cultured sympathetic neurons from the superior cervical ganglia of E21 Sprague-Dawley rat pups following growth under control conditions or after treatment with BMP-7 at 50 ng/mL for 6 hours or 24 hours.
Project description:We examined gene expression profiles (27028 genes) in the livers of Sprague-Dawley rats with no infection and at 2 and 4 weeks after Clonorchis sinensis infection using Whole Rat Genome Microarray 4x44K v3 (GPL14745, Agilent-028282)
Project description:The aim is to characterize rat liver fibrosis induced by bile duct ligation (BDL). To induce hepatic fibrosis, Male Sprague Dawley rats (9-12 weeks of age and 380-420 g of weight upon arrival, supplied by Beijing Vital River laboratory animal Co., Ltd.) underwent surgery of bile duct ligation (BDL). The bile ducts of Sprague-Dawley rats were ligated after 12 hours of fasting and water deprivation. Rat liver samples were collected from three groups of rats at week 1, 2 and 5 after BDL surgery. Three control groups of rats underwent sham operation, including bile duct mobilization, but without BDL. Three biological replicates were used for each group.