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Whole genome analysis in A549 cells treated for short incubation periods with various concentrations of triptolide

ABSTRACT: The purpose of this study was to identify the genes primarily affected by a short treatment of A549 cells with grading concentrations of the natural compound triptolide. The cells were exposed to this molecule before RNA was extracted, then used for whole human genome microarray experiments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE16760 | GEO | 2009/10/16

SECONDARY ACCESSION(S): PRJNA117409

REPOSITORIES: GEO

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Whole genome analysis in A549 cells treated for short incubation periods with various concentrations of triptolide

Project description:The purpose of this study was to identify the genes primarily affected by a short treatment of A549 cells with grading concentrations of the natural compound triptolide. The cells were exposed to this molecule before RNA was extracted, then used for whole human genome microarray experiments. 12 samples, corresponding to untreated cells plus three concentrations (0.05, 0.15, 0.45 ÂµM) of tripolide for each time point (1, 2, and 4 hours). Each sample was analysed in triplicate, i.e. three microarray for each condition, making a total of 36 microarrays.

2010-06-21 | E-GEOD-16760 | biostudies-arrayexpress

Whole genome analysis in A549 cells treated for short incubation periods with various concentrations of triptolide

Project description:The purpose of this study was to identify the genes primarily affected by a short treatment of A549 cells with grading concentrations of the natural compound triptolide. The cells were exposed to this molecule before RNA was extracted, then used for whole human genome microarray experiments. 12 samples, corresponding to untreated cells plus three concentrations (0.05, 0.15, 0.45 µM) of tripolide for each time point (1, 2, and 4 hours). Each sample was analysed in triplicate, i.e. three microarray for each condition, making a total of 36 microarrays.

| S-ECPF-GEOD-16760 | biostudies-other

Dysregulation of lncRNAs and circRNAs in mouse testes after exposure to triptolide

Project description:Triptolide has been shown to exert various pharmacological effects on systemic autoimmune diseases and cancers. However, its severe toxicity, especially reproduction toxicity, prevents wide clinical use for those people with fertility needs. Noncoding RNAs including lncRNAs and cicrRNAs are regulatory molecules that mediate a wide variety of physiological activities, which are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide-induced reproduction toxicity is fully unknown. Here, after exposure of mice to triptolide for 35 day, the total RNAs were used to investigate lncRNAs/cicrRNAs/mRNAs expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide-induced toxicity. The results showed that triptolide significantly decreased the testicular weight, damaged testis and sperm morphology, reduced sperm motility and density. Furthermore, there's a remarkable deformity in sperm head and tail in triptolide-exposed mice. At the transcriptome level, the triptolide-treated mice exhibited aberrant expression profiles for lncRNAs/cicrRNAs/mRNAs, many of them were dysregulated. Gene ontology and pathway analyses revealed that the functions of differentially expressed lncRNA targets, cicrRNAs cognate genes and mRNAs were closely linked with many processes involved in spermatogenesis. Additionally, many newly identified lncRNAs /cicrRNAs displayed inducible expression, suggesting that they might be good candidate markers for triptolide-induced male reproductive toxicity. This study gives us an insight into understanding the reproductive system toxicity of triptolide and reminds us to strengthen the research on increasing efficacy and decreasing toxicity of triptolide.

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The genes affected by Triptolide in activated mouse primary T cells

Project description:Triptolide possesses immunosuppressive activity and its efficacy is commonly believed to be related to the suppression of T cells. However, the underlying mechanism is still not well documented and most of the previous study adopted tumor cell lines of immune system as model cells in mechanism research, these cells’ signal transduction pathway can not reflect the real situation of immune system. In this study, we investigated the immunosuppressive mechanism of triptolide on T cell activation and proliferation using mouse primary T lymphocytes. These effects were analyzed along with activation of the TCR and AP-1 pathway. We used microarrays to detail the global programme of gene expression underlying stimulation and triptolide treatment. Mouse primary T cells stimulated with anti-CD3/CD28 antibodies treated with triptolide or not treated with triptolide were selected for RNA extraction and hybridization on Affymetrix microarrays.

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Transcriptome of cancer associated fibroblasts before and after triptolide treatment in pancreatic cancer

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Project description:Chronic inflammation plays important role in lung cancer development. Recently, we found that anti-inflammation drugs aspirin and triptolide when combined showed synergistic effect in suppressing lung cancer development. In this study, we aim to use gene microarray to define the genes and pathways that are affected by aspirin, triptolide individually or in combination.

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Expression data from treatment of actinomycin D and triptolide on MCF7 cells

Project description:Expression data from treatment of actinomycin D (2.5uM) and triptolide (500 nM) on MCF7 cells for 2, 4 and 6 hours. We used microarrays to explore the mechanism of triptolide action. MCF7 cells were treated with actinomycin D or triptolide for 2, 4 and 6 hours, RNA was extracted and hybridization on Affymetrix microarrays. Data were normalized by RMA.

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