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Expression data from treatment of actinomycin D and triptolide on MCF7 cells

ABSTRACT: Expression data from treatment of actinomycin D (2.5uM) and triptolide (500 nM) on MCF7 cells for 2, 4 and 6 hours. We used microarrays to explore the mechanism of triptolide action.

ORGANISM(S): Homo sapiens

PROVIDER: GSE28662 | GEO | 2011/10/18

SECONDARY ACCESSION(S): PRJNA138867

REPOSITORIES: GEO

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Expression data from treatment of actinomycin D and triptolide on MCF7 cells

Project description:Expression data from treatment of actinomycin D (2.5uM) and triptolide (500 nM) on MCF7 cells for 2, 4 and 6 hours. We used microarrays to explore the mechanism of triptolide action. MCF7 cells were treated with actinomycin D or triptolide for 2, 4 and 6 hours, RNA was extracted and hybridization on Affymetrix microarrays. Data were normalized by RMA.

2011-10-17 | E-GEOD-28662 | biostudies-arrayexpress

HCT116 cells isogenic for p53 expression upon treatment with actinomycin D and nutlin-3

2009-09-01 | GSE12459 | GEO

Triptolide alters the miRNA expression profiles in human hepatocellular carcinoma HepG2 cells

Project description:We compared miRNA expression profiles among 5 groups of HepG2 cells treated with various concentrations (0,100,200 nM) of triptolide for 12 h or 24 h.

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Triptolide alters the miRNA expression profiles in human hepatocellular carcinoma HepG2 cells

Project description:We compared miRNA expression profiles among 5 groups of HepG2 cells treated with various concentrations (0,100,200 nM) of triptolide for 12 h or 24 h. Two-condition experiment, triptolide intervention vs. without intervention. Biological replicates: 3 control, 3 treated, independently grown and harvested. One replicate per array.

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Transcriptome of cancer associated fibroblasts before and after triptolide treatment in pancreatic cancer

Project description:Pancreatic cancer is characterized by heavy desmoplasia. Triptolide and its water-soluble pro-drug Minnelide are extremely efficient against pancreatic cancer in animal models. However, the effects of triptolide on pancreatic cancer stromal cells are largely unknown. The aim of this project is to indentify potential cellular functions that are affected by triptolide in pancreatic cancer associated fibroblasts.

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Gene expression change after Enzalutamide treatment in MCF7 cells

Project description:To explore the mechanism of action of Enzalutamide, we performed RNA-seq to investigate gene expression difference after Enzalutamide treatment in both MCF7 and MCF7 with AR over expression (AROE) cells. RNA-sequencing (RNA-seq) of MCF7 and MCF7 AROE cells with DMSO or Enzalutamide treatment

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HCT116 cells isogenic for p53 expression upon treatment with actinomycin D and nutlin-3

Project description:HCT116 wild type cells express p53. The isogenic HCT116 p53KO cells have the p53 gene knocked out. Cells were treated for 16 hours. Dosages for actinomycin D treatment was 1 nM, 10 nM, and 100 nM. Dosage for nutlin-3 was 4 uM. Keywords: dose response Measuring the gene expression profile of the isogenic cell lines when the cells were treated with 2 different drugs at different concentrations

2010-06-24 | E-GEOD-12459 | biostudies-arrayexpress

HEK 293T LCMS after 8h of treatment with Actinomycin D

Project description:Investigation of proteomic changes in HEK 293T cells after 8h of actinomycin D treatment with special focus on RNA modifying enzymes.

2023-07-20 | PXD039549 | Pride

Homo sapiens

Project description:Expression data from treatment of actinomycin D and triptolide on MCF7 cells

| PRJNA138867 | ENA

Dysregulation of lncRNAs and circRNAs in mouse testes after exposure to triptolide

Project description:Triptolide has been shown to exert various pharmacological effects on systemic autoimmune diseases and cancers. However, its severe toxicity, especially reproduction toxicity, prevents wide clinical use for those people with fertility needs. Noncoding RNAs including lncRNAs and cicrRNAs are regulatory molecules that mediate a wide variety of physiological activities, which are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide-induced reproduction toxicity is fully unknown. Here, after exposure of mice to triptolide for 35 day, the total RNAs were used to investigate lncRNAs/cicrRNAs/mRNAs expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide-induced toxicity. The results showed that triptolide significantly decreased the testicular weight, damaged testis and sperm morphology, reduced sperm motility and density. Furthermore, there's a remarkable deformity in sperm head and tail in triptolide-exposed mice. At the transcriptome level, the triptolide-treated mice exhibited aberrant expression profiles for lncRNAs/cicrRNAs/mRNAs, many of them were dysregulated. Gene ontology and pathway analyses revealed that the functions of differentially expressed lncRNA targets, cicrRNAs cognate genes and mRNAs were closely linked with many processes involved in spermatogenesis. Additionally, many newly identified lncRNAs /cicrRNAs displayed inducible expression, suggesting that they might be good candidate markers for triptolide-induced male reproductive toxicity. This study gives us an insight into understanding the reproductive system toxicity of triptolide and reminds us to strengthen the research on increasing efficacy and decreasing toxicity of triptolide.

2020-03-23 | GSE126857 | GEO

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