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Bioengineered embryoids mimic post-implantation development in vitro [EmbryoidsTruseq3]

ABSTRACT: Here, we report a bioengineering-inspired approach to simultaneously coax mouse embryonic stem cells (ESCs) into hundreds of uniform epiblast-like (EPI) aggregates in a solid matrix-free manner. When co-cultured with mouse trophoblast stem cell (TSC) aggregates, the resulting hybrid structures initiate gastrulation-like events and undergo axial morphogenesis to yield structures, termed EpiTS embryoids, with a pronounced anterior development, including brain-like regions.

ORGANISM(S): Mus musculus

PROVIDER: GSE171211 | GEO | 2021/04/12

REPOSITORIES: GEO

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Bioengineered embryoids mimic post-implantation development in vitro [Embryoids10XG]

Project description:Here, we report a bioengineering-inspired approach to simultaneously coax mouse embryonic stem cells (ESCs) into hundreds of uniform epiblast-like (EPI) aggregates in a solid matrix-free manner. When co-cultured with mouse trophoblast stem cell (TSC) aggregates, the resulting hybrid structures initiate gastrulation-like events and undergo axial morphogenesis to yield structures, termed EpiTS embryoids, with a pronounced anterior development, including brain-like regions.

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Bioengineered embryoids mimic post-implantation development in vitro [EmbryoidsTruseq3]

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Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development

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