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Cut & Run mapping the alteration of H4K20me1 and transcription activity in cultured MuSC upon Suv420h1 depletion.

ABSTRACT: Cut & Run mapping the alteration of H4K20me1 and transcription activity in cultured MuSC upon Suv420h1 depletion.

PROVIDER: PRJNA724985 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14320700_1.fastq.gz	Fastqsanger.gz
	SRR14320700_2.fastq.gz	Fastqsanger.gz
	SRR14320701_1.fastq.gz	Fastqsanger.gz
	SRR14320701_2.fastq.gz	Fastqsanger.gz
	SRR14320702_1.fastq.gz	Fastqsanger.gz

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Cut & Run mapping the alteration of H4K20me1 and transcription activity in cultured MuSC upon Suv420h1 depletion.

Project description:H4K20me1/2/3 is dynamically regulated during cell cycle to maintain the genomic stability. Here, we found that depletion of H4K20 di-methyltransferase Suv420h1 in mouse adult MuSCs leads to strong accumulation of H4K20me1 specifically in S phase. In response to it, the transcription activity is increased upon Suv420h1 depletion. To investigate which genomic regions and how the transcription are regulated by Suv420h1 in MuSC, we performed Cut&Run of H4K20me1 and RNA polymerase II Ser2P in control and Suv420h1 inactivated MuSCs.

2022-10-13 | GSE173259 | GEO

DRIP-seq mapping the R-loop formation in cultured Ctrl and Suv420h1 mutant MuSC

Project description:Inactivation of Suv420h1 in MuSC lead to increased transcription and replication collision thereby caused accumulation of RNA:DNA hybrids. This study profiles the R-loops in Ctrl and Suv420h1m mutant MuSCs.

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Genome-wide maps of nascent transcriptions in proliferating Ctrl and Suv420h1 mutant muscle stem cells

Project description:To profile the nascent transcription activity including coding genes and eRNAs, we performed precision nuclear run-on sequencing (PRO-Seq) in control and Suv420h1 mutant MuSC

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DRIP-seq mapping the R-loop formation in cultured Ctrl and Suv420h1 mutant MuSC

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Expression data from Suv420h1 knockout and mutant PREX2 expressing MEFs (mouse embryonic fibroblasts)

Project description:PREX2 truncating mutations occur in melanoma. We used microarray based gene expression profiling to compare expression patterns between cells harboring Suv420h1 knockout and PREX2 mutant expressing Spontaneously immortalized MEFs from either wt or Suv420h1 knockout mice were used. Wt MEFs were transduced with lentivirus to express indicated PREX2 mutants. Suv420h1 ko MEFs were reconstituted either with control GFP or Suv420h1-GFP fusion. Total RNA was isolated and subjected to analysis

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Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase

Project description:Here we report the cryo-electron microscopy (cryo-EM) structures of SUV420H1 associated with canonical nucleosome core particles (NCPs) or H2AZ containing NCPs. We find that SUV420H1 shows conformational change after bound to a nucleosome and make extensive site-specific contacts with histone and DNA regions, thus enabling H4K20 insertion for catalysis specifically. Through in vivo functional analysis, we validated special residues of SUV420H1 that are critical for its catalytic activity and function in chromatin state regulation.Thus, our study provides molecular insights into the nucleosome-based recognition and histone- methylation mechanisms of SUV420H1.

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CUT&RUN analysis for NOP16-mediated H3K27me3 alteration in MDA-MB231 cells [CUT&RUN-seq]

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