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RedChIP identifies noncoding RNAs associated with genomic sites occupied by Polycomb and CTCF proteins


ABSTRACT: Nuclear noncoding RNAs are key regulators of gene expression and chromatin organization. Here we present RedChIP, a technique combining RNA-DNA proximity ligation and chromatin immunoprecipitation for identification of RNAs associated with genomic regions occupied by a protein of interest. We identify a spectrum of cis and trans acting lincRNAs enriched at Polycomb and CTCF binding sites, which may be involved in Polycomb mediated gene repression and chromatin looping.

ORGANISM(S): Homo sapiens

PROVIDER: GSE174474 | GEO | 2021/12/18

REPOSITORIES: GEO

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